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Interaction of two flavonols with fat mass and obesity-associated protein investigated by fluorescence quenching and molecular docking.
Wang, Zechun; Wang, Ning; Han, Xinxin; Wang, Ruiyong; Chang, Junbiao.
Afiliação
  • Wang Z; a College of Chemistry and Molecular Engineering , Zhengzhou University , 100 Science Avenue, Zhengzhou 450001 , China.
  • Wang N; a College of Chemistry and Molecular Engineering , Zhengzhou University , 100 Science Avenue, Zhengzhou 450001 , China.
  • Han X; a College of Chemistry and Molecular Engineering , Zhengzhou University , 100 Science Avenue, Zhengzhou 450001 , China.
  • Wang R; a College of Chemistry and Molecular Engineering , Zhengzhou University , 100 Science Avenue, Zhengzhou 450001 , China.
  • Chang J; a College of Chemistry and Molecular Engineering , Zhengzhou University , 100 Science Avenue, Zhengzhou 450001 , China.
J Biomol Struct Dyn ; 36(13): 3388-3397, 2018 Oct.
Article em En | MEDLINE | ID: mdl-28978264
ABSTRACT
The binding of two flavonols with fat mass and obesity-associated protein (FTO) was studied using fluorescence spectroscopy, Stern-Volmer kinetics, UV-Vis absorption, and molecular docking. The quenching of FTO fluorescence was determined to be static with binding constants on the order of 104 M-1. The interaction was studied over three temperatures, and the binding was found to be exothermic with a positive change in entropy. Thermodynamic analysis and molecular modeling suggest that hydrophobic interaction and hydrogen bonding interaction are the main binding force in stabilizing the flavonol-FTO complex.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Flavonóis / Interações Hidrofóbicas e Hidrofílicas / Dioxigenase FTO Dependente de alfa-Cetoglutarato Tipo de estudo: Risk_factors_studies Idioma: En Revista: J Biomol Struct Dyn Ano de publicação: 2018 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Flavonóis / Interações Hidrofóbicas e Hidrofílicas / Dioxigenase FTO Dependente de alfa-Cetoglutarato Tipo de estudo: Risk_factors_studies Idioma: En Revista: J Biomol Struct Dyn Ano de publicação: 2018 Tipo de documento: Article País de afiliação: China