Ectopic expression of Pax4 in pancreatic δ cells results in ß-like cell neogenesis.
J Cell Biol
; 216(12): 4299-4311, 2017 12 04.
Article
em En
| MEDLINE
| ID: mdl-29025873
ABSTRACT
The recent demonstration that pancreatic α cells can be continuously regenerated and converted into ß-like cells upon ectopic expression of Pax4 opened new avenues of research in the endocrine cell differentiation and diabetes fields. To determine whether such plasticity was also shared by δ cells, we generated and characterized transgenic animals that express Pax4 specifically in somatostatin-expressing cells. We demonstrate that the ectopic expression of Pax4 in δ cells is sufficient to induce their conversion into functional ß-like cells. Importantly, this conversion induces compensatory mechanisms involving the reactivation of endocrine developmental processes that result in dramatic ß-like cell hyperplasia. Importantly, these ß-like cells are functional and can partly reverse the consequences of chemically induced diabetes.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Homeodomínio
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Células Secretoras de Somatostatina
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Diabetes Mellitus Experimental
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Células Secretoras de Insulina
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Fatores de Transcrição Box Pareados
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Expressão Ectópica do Gene
Limite:
Animals
Idioma:
En
Revista:
J Cell Biol
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
França