High-throughput, image-based screening of pooled genetic-variant libraries.
Nat Methods
; 14(12): 1159-1162, 2017 Dec.
Article
em En
| MEDLINE
| ID: mdl-29083401
ABSTRACT
We report a high-throughput screening method that allows diverse genotypes and corresponding phenotypes to be imaged in individual cells. We achieve genotyping by introducing barcoded genetic variants into cells as pooled libraries and reading the barcodes out using massively multiplexed fluorescence in situ hybridization. To demonstrate the power of image-based pooled screening, we identified brighter and more photostable variants of the fluorescent protein YFAST among 60,000 variants.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biblioteca Gênica
/
Hibridização in Situ Fluorescente
/
Escherichia coli
/
Código de Barras de DNA Taxonômico
/
Sequenciamento de Nucleotídeos em Larga Escala
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Idioma:
En
Revista:
Nat Methods
Assunto da revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Estados Unidos