A rapid and simple HPLC-FLD screening method with QuEChERS as the sample treatment for the simultaneous monitoring of nine bisphenols in milk.

ABSTRACT

A specific, precise and accurate high performance liquid chromatographic (HPLC) analytical method with a fluorescence detector (FLD) was established for the simultaneous determination of nine bisphenols (BPs) in milk samples. Samples were extracted ultrasonically with acetonitrile and cleaned using the QuEChERS technique. Under the optimized conditions, good linearity was obtained for the nine BPs and the correlation coefficients (R2) ranged from 0.9942 to 0.9997. Recovery values for the nine bisphenols in spiked samples were 75.82-93.86% with intra-day and inter-day relative standard deviations (RSDs) from 2.6 to 11.1%. The limits of detection (LODs) and quantification (LOQs) were 1.0-3.1 µg/kg and 3.5-9.8 µg/kg, respectively. The results demonstrated clearly that the approach developed provides reliable, simple, rapid and environmentally-friendly quantification and identification of nine bisphenols in a fatty matrix and could be used for monitoring bisphenols in milk.