Simultaneous determination of fast and slow dynamics in molecules using extreme CPMG relaxation dispersion experiments.
J Biomol NMR
; 70(1): 1-9, 2018 01.
Article
em En
| MEDLINE
| ID: mdl-29188417
ABSTRACT
Molecular dynamics play a significant role in how molecules perform their function. A critical method that provides information on dynamics, at the atomic level, is NMR-based relaxation dispersion (RD) experiments. RD experiments have been utilized for understanding multiple biological processes occurring at micro-to-millisecond time, such as enzyme catalysis, molecular recognition, ligand binding and protein folding. Here, we applied the recently developed high-power RD concept to the Carr-Purcell-Meiboom-Gill sequence (extreme CPMG; E-CPMG) for the simultaneous detection of fast and slow dynamics. Using a fast folding protein, gpW, we have shown that previously inaccessible kinetics can be accessed with the improved precision and efficiency of the measurement by using this experiment.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Modelos Moleculares
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Ressonância Magnética Nuclear Biomolecular
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Simulação de Dinâmica Molecular
Idioma:
En
Revista:
J Biomol NMR
Assunto da revista:
BIOLOGIA MOLECULAR
/
DIAGNOSTICO POR IMAGEM
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MEDICINA NUCLEAR
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Alemanha