The RNA-binding protein PTBP1 is necessary for B cell selection in germinal centers.
Nat Immunol
; 19(3): 267-278, 2018 03.
Article
em En
| MEDLINE
| ID: mdl-29358707
ABSTRACT
Antibody affinity maturation occurs in germinal centers (GCs), where B cells cycle between the light zone (LZ) and the dark zone. In the LZ, GC B cells bearing immunoglobulins with the highest affinity for antigen receive positive selection signals from helper T cells, which promotes their rapid proliferation. Here we found that the RNA-binding protein PTBP1 was needed for the progression of GC B cells through late S phase of the cell cycle and for affinity maturation. PTBP1 was required for proper expression of the c-MYC-dependent gene program induced in GC B cells receiving T cell help and directly regulated the alternative splicing and abundance of transcripts that are increased during positive selection to promote proliferation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos B
/
Ativação Linfocitária
/
Centro Germinativo
/
Proteína de Ligação a Regiões Ricas em Polipirimidinas
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Ribonucleoproteínas Nucleares Heterogêneas
/
Seleção Clonal Mediada por Antígeno
Limite:
Animals
Idioma:
En
Revista:
Nat Immunol
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Reino Unido