Long-term preservation of planar cell polarity in reversed tracheal epithelium.
Respir Res
; 19(1): 22, 2018 02 02.
Article
em En
| MEDLINE
| ID: mdl-29394896
ABSTRACT
BACKGROUND:
Planar cell polarity (PCP) coordinates the patterning and orientation of cells and their structures along tissue planes, and although its acquisition during the formation of airway epithelium has been described, the mechanisms for its maintenance and reconstruction are poorly understood. We aimed to clarify whether ambient environment change by orthotropic autologous transplantation affected PCP at the cellular level.METHODS:
We performed orthotropic autologous transplantation by inverting tracheal segments in rats, and then performed morphological evaluation by microscopy. The PCP of the tracheal epithelium was assessed over time by analyzing the directions of mucociliary transport and ciliary beat, the positional relationship between the basal body and basal foot, and the bias of Vang-like protein 1 (Vangl1) at 2, 4, and 6 months postoperatively.RESULTS:
After 2 months, the directions of mucociliary transport and ciliary beat were preserved toward the lung in the inverted tracheal segments. The positional relationship between the basal body and the basal foot, and the bias of Vangl1, also indicated preservation of PCP in the inverted tracheal segments. Similar results were obtained at 6 months.CONCLUSION:
The PCP of ciliated epithelium was preserved in reversed trachea, even after long-term observation.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Traqueia
/
Polaridade Celular
/
Mucosa Respiratória
Limite:
Animals
Idioma:
En
Revista:
Respir Res
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Japão