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Novel, diverse RNA viruses from Mediterranean isolates of the phytopathogenic fungus, Rosellinia necatrix: insights into evolutionary biology of fungal viruses.
Arjona-Lopez, Juan Manuel; Telengech, Paul; Jamal, Atif; Hisano, Sakae; Kondo, Hideki; Yelin, Mery Dafny; Arjona-Girona, Isabel; Kanematsu, Satoko; Lopez-Herrera, Carlos José; Suzuki, Nobuhiro.
Afiliação
  • Arjona-Lopez JM; Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046, Japan.
  • Telengech P; Instituto de Agricultura Sostenible C.S.I.C., Alameda del Obispo, s/n. CP: 14004, Cordoba, Spain.
  • Jamal A; Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046, Japan.
  • Hisano S; Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046, Japan.
  • Kondo H; Crop Diseases Research Institute, National Agricultural Research Centre, Islamabad 45500, Pakistan.
  • Yelin MD; Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046, Japan.
  • Arjona-Girona I; Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046, Japan.
  • Kanematsu S; Northern Agriculture Research & Development, Migal Galilee Technology Center, P.O.B. 831, Kiryat Shemona 11016, Israel.
  • Lopez-Herrera CJ; Instituto de Agricultura Sostenible C.S.I.C., Alameda del Obispo, s/n. CP: 14004, Cordoba, Spain.
  • Suzuki N; NARO Headquarter, National Agriculture and Food Research Organization (NARO), 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8517, Japan.
Environ Microbiol ; 20(4): 1464-1483, 2018 04.
Article em En | MEDLINE | ID: mdl-29411500
To reveal mycovirus diversity, we conducted a search of as-yet-unexplored Mediterranean isolates of the phytopathogenic ascomycete Rosellinia necatrix for virus infections. Of seventy-nine, eleven fungal isolates tested RNA virus-positive, with many showing coinfections, indicating a virus incidence of 14%, which is slightly lower than that (approximately 20%) previously reported for extensive surveys of over 1000 Japanese R. necatrix isolates. All viral sequences were fully or partially characterized by Sanger and next-generation sequencing. These sequences appear to represent isolates of various new species spanning at least 6 established or previously proposed families such as Partiti-, Hypo-, Megabirna-, Yado-kari-, Fusagra- and Fusarividae, as well as a newly proposed family, Megatotiviridae. This observation greatly expands the diversity of R. necatrix viruses, because no hypo-, fusagra- or megatotiviruses were previously reported from R. necatrix. The sequence analyses showed a rare horizontal gene transfer event of the 2A-like protease domain between a dsRNA (phlegivirus) and a positive-sense, single-stranded RNA virus (hypovirus). Moreover, many of the newly detected viruses showed the closest relation to viruses reported from fungi other than R. necatrix, such as Fusarium spp., which are sympatric to R. necatrix. These combined results imply horizontal virus transfer between these soil-inhabitant fungi.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírus de RNA / Xylariales / Micovírus País/Região como assunto: Europa Idioma: En Revista: Environ Microbiol Assunto da revista: MICROBIOLOGIA / SAUDE AMBIENTAL Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Japão

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírus de RNA / Xylariales / Micovírus País/Região como assunto: Europa Idioma: En Revista: Environ Microbiol Assunto da revista: MICROBIOLOGIA / SAUDE AMBIENTAL Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Japão