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Single cell RNA sequencing of stem cell-derived retinal ganglion cells.
Daniszewski, Maciej; Senabouth, Anne; Nguyen, Quan H; Crombie, Duncan E; Lukowski, Samuel W; Kulkarni, Tejal; Sluch, Valentin M; Jabbari, Jafar S; Chamling, Xitiz; Zack, Donald J; Pébay, Alice; Powell, Joseph E; Hewitt, Alex W.
Afiliação
  • Daniszewski M; Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne 3002, Australia.
  • Senabouth A; Ophthalmology, Department of Surgery, the University of Melbourne, Melbourne 3002, Australia.
  • Nguyen QH; Institute for Molecular Bioscience, University of Queensland, St Lucia, Brisbane 4067, Australia.
  • Crombie DE; Institute for Molecular Bioscience, University of Queensland, St Lucia, Brisbane 4067, Australia.
  • Lukowski SW; Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne 3002, Australia.
  • Kulkarni T; Ophthalmology, Department of Surgery, the University of Melbourne, Melbourne 3002, Australia.
  • Sluch VM; Institute for Molecular Bioscience, University of Queensland, St Lucia, Brisbane 4067, Australia.
  • Jabbari JS; Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne 3002, Australia.
  • Chamling X; Ophthalmology, Department of Surgery, the University of Melbourne, Melbourne 3002, Australia.
  • Zack DJ; Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
  • Pébay A; Australian Genome Research Facility, Melbourne 3051, Australia.
  • Powell JE; Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
  • Hewitt AW; Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
Sci Data ; 5: 180013, 2018 02 13.
Article em En | MEDLINE | ID: mdl-29437159
ABSTRACT
We used single cell sequencing technology to characterize the transcriptomes of 1,174 human embryonic stem cell-derived retinal ganglion cells (RGCs) at the single cell level. The human embryonic stem cell line BRN3B-mCherry (A81-H7), was differentiated to RGCs using a guided differentiation approach. Cells were harvested at day 36 and prepared for single cell RNA sequencing. Our data indicates the presence of three distinct subpopulations of cells, with various degrees of maturity. One cluster of 288 cells showed increased expression of genes involved in axon guidance together with semaphorin interactions, cell-extracellular matrix interactions and ECM proteoglycans, suggestive of a more mature RGC phenotype.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células Ganglionares da Retina / RNA / Células-Tronco Embrionárias Limite: Humans Idioma: En Revista: Sci Data Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Austrália
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células Ganglionares da Retina / RNA / Células-Tronco Embrionárias Limite: Humans Idioma: En Revista: Sci Data Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Austrália