Impact of type 1 diabetes mellitus and sitagliptin treatment on the neuropeptide Y system of rat retina.
Clin Exp Ophthalmol
; 46(7): 783-795, 2018 09.
Article
em En
| MEDLINE
| ID: mdl-29442423
ABSTRACT
BACKGROUND:
Neuropeptide Y (NPY) is a neuromodulator that is expressed in the retina. Increasing evidence suggests that NPY has pronounced anti-inflammatory effects, which might depend on the inhibition of dipeptidyl-peptidase-IV (DPP-IV). The aim of this study was to investigate the impact of type 1 diabetes mellitus (DM) and sitagliptin, a DPP-IV inhibitor, on the NPY system in the retina using an animal model.METHODS:
Type 1 DM was induced in male Wistar rats by an intraperitoneal injection of streptozotocin. Starting 2 weeks after DM onset, animals were treated orally with sitagliptin (5 mg/kg.day) for 2 weeks. The expression of NPY and NPY receptors (Y1 , Y2 and Y5 receptors) was measured by quantitative polymerase chain reaction, Western blot and/or enzyme-linked immunosorbent assay. The immunoreactivity of NPY and NPY receptors was evaluated by immunohistochemistry, and the [35 S]GTPγS binding assay was used to assess the functional binding of NPY receptors.RESULTS:
DM decreased the mRNA levels of NPY in the retina, as well as the protein levels of NPY and Y5 receptor. No changes were detected in the localization of NPY and NPY receptors in the retina and in the functional binding of NPY to all receptors. Sitagliptin alone reduced retinal NPY mRNA levels. The effects of DM on the NPY system were not affected by sitagliptin.CONCLUSION:
DM modestly affects the NPY system in the retina and these effects are not prevented by sitagliptin treatment. These observations suggest that DPP-IV enzyme is not underlying the NPY changes detected in the retina induced by type 1 DM.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Retina
/
Neuropeptídeo Y
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Regulação da Expressão Gênica
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Diabetes Mellitus Experimental
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Diabetes Mellitus Tipo 1
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Retinopatia Diabética
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Fosfato de Sitagliptina
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
Clin Exp Ophthalmol
Assunto da revista:
OFTALMOLOGIA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Portugal