Elevation of autoantibody level against PDCD11 in patients with transient ischemic attack.
Oncotarget
; 9(10): 8836-8848, 2018 Feb 06.
Article
em En
| MEDLINE
| ID: mdl-29507658
ABSTRACT
BACKGROUND:
Disease specific autoantibodies have been detected in the sera of patients with atherosclerosis-related diseases, such as cerebral infarction, cardiovascular disease. In the present study, we aimed to identify novel autoantibodies responsible for transient ischemic attack (TIA), a prodromal condition for cerebral infarction.METHODS:
To identify candidate antigens, we screened a human aortic endothelial cell cDNA library using sera from 20 patients with TIA. Serum antibody levels were measured using amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) in 2 independent patient/healthy donor (HD) cohorts (n = 192 and n = 906 in the second screening and validation cohort, respectively).RESULTS:
First screening identified 3 candidate antigens. Of these, programmed cell death 11 (PDCD11) was determined to be associated with stroke (p < 0.0001), as evidenced from the second screening using AlphaLISA. The validation cohort revealed significantly higher antibody levels against PDCD11 (PDCD11-Ab levels) in patients with TIA than in HDs. Multivariate logistic regression analysis indicated that the predictive value of PDCD11-Ab levels for TIA [Odds ratio (OR) 2.44, 95% confidence interval (CI) 1.33-4.57, p = 0.0039] was not inferior to other known risk factors for ischemic stroke, including age (OR 4.97, 95% CI 2.67-9.48, p < 0.0001); hypertension (OR 3.21, 95% CI 1.76-5.86, p = 0.0001); and diabetes (OR 4.31, 95% CI 1.74-11.2, p = 0.0015).CONCLUSION:
Serum PDCD11-Ab level may serve as a potential biomarker for TIA.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Idioma:
En
Revista:
Oncotarget
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Japão