Reproducible proteomics sample preparation for single FFPE tissue slices using acid-labile surfactant and direct trypsinization.

Föll, Melanie Christine; Fahrner, Matthias; Oria, Victor Oginga; Kühs, Markus; Biniossek, Martin Lothar; Werner, Martin; Bronsert, Peter; Schilling, Oliver

Föll, Melanie Christine; Fahrner, Matthias; Oria, Victor Oginga; Kühs, Markus; Biniossek, Martin Lothar; Werner, Martin; Bronsert, Peter; Schilling, Oliver.

Afiliação

Föll MC; 1Institute of Molecular Medicine and Cell Research, Faculty of Medicine, University of Freiburg, Stefan Meier Strasse 17, 79104 Freiburg, Germany.
Fahrner M; 2Faculty of Biology, Albert-Ludwigs-University Freiburg, Freiburg, Germany.
Oria VO; 1Institute of Molecular Medicine and Cell Research, Faculty of Medicine, University of Freiburg, Stefan Meier Strasse 17, 79104 Freiburg, Germany.
Kühs M; 2Faculty of Biology, Albert-Ludwigs-University Freiburg, Freiburg, Germany.
Biniossek ML; 3Spemann Graduate School of Biology and Medicine (SGBM), Albert-Ludwigs-University Freiburg, Freiburg, Germany.
Werner M; 1Institute of Molecular Medicine and Cell Research, Faculty of Medicine, University of Freiburg, Stefan Meier Strasse 17, 79104 Freiburg, Germany.
Bronsert P; 2Faculty of Biology, Albert-Ludwigs-University Freiburg, Freiburg, Germany.
Schilling O; 3Spemann Graduate School of Biology and Medicine (SGBM), Albert-Ludwigs-University Freiburg, Freiburg, Germany.

Clin Proteomics ; 15: 11, 2018.

Article em En | MEDLINE | ID: mdl-29527141

Palavras-chave

Archival tissue; Biomarker discovery; FASP; FFPE; Label-free quantitation; Mass spectrometry; Proteomics; RapiGest; Stained tissue

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Guideline / Qualitative_research Idioma: En Revista: Clin Proteomics Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Alemanha

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google