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Time-lapse observation of stepwise regression of Erk activity in zebrafish presomitic mesoderm.
Sari, Dini Wahyu Kartika; Akiyama, Ryutaro; Naoki, Honda; Ishijima, Hannosuke; Bessho, Yasumasa; Matsui, Takaaki.
Afiliação
  • Sari DWK; Gene Regulation Research, Nara Institute of Science and Technology, 8916-5 Takayama, Nara, 630-0101, Japan.
  • Akiyama R; Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Jl. Flora, Bulaksumur, Yogyakarta, 55281, Indonesia.
  • Naoki H; Gene Regulation Research, Nara Institute of Science and Technology, 8916-5 Takayama, Nara, 630-0101, Japan.
  • Ishijima H; Laboratory of Theoretical Biology, Graduate School of Biostudies, Kyoto University, Yoshidakonoecho, Sakyo, Kyoto, 606-8315, Japan.
  • Bessho Y; Gene Regulation Research, Nara Institute of Science and Technology, 8916-5 Takayama, Nara, 630-0101, Japan.
  • Matsui T; Gene Regulation Research, Nara Institute of Science and Technology, 8916-5 Takayama, Nara, 630-0101, Japan.
Sci Rep ; 8(1): 4335, 2018 03 12.
Article em En | MEDLINE | ID: mdl-29531317
ABSTRACT
During somite segmentation, clock genes oscillate within the posterior presomitic mesoderm (PSM). The temporal information ties up with the posteriorly moving FGF gradient, leading to the formation of a presumptive somite within the PSM. We previously investigated Erk activity downstream of FGF signaling by collecting stained zebrafish embryos, and discovered that the steep gradient of Erk activity was generated in the PSM, and the Erk activity border regularly shifted in a stepwise manner. However, since these interpretations come from static analyses, we needed to firmly confirm them by applying an analysis that has higher spatiotemporal resolutions. Here we developed a live imaging system for Erk activity in zebrafish embryos, using a Förster resonance energy transfer (FRET)-based Erk biosensor. With this system, we firmly showed that Erk activity exhibits stepwise regression within the PSM. Although our static analyses could not detect the stepwise pattern of Erk activity in clock-deficient embryos, our system revealed that, in clock-deficient embryos, the stepwise regression of Erk activity occurs at an irregular timing, eventually leading to formation of irregularly-sized somites. Therefore, our system overcame the limitation of static analyses and revealed that clock-dependent spatiotemporal regulation of Erk is required for proper somitogenesis in zebrafish.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peixe-Zebra / Somitos / Sistema de Sinalização das MAP Quinases / Proteínas de Peixe-Zebra Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Japão

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peixe-Zebra / Somitos / Sistema de Sinalização das MAP Quinases / Proteínas de Peixe-Zebra Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Japão