The Phosphatase PTPL1 Is Required for PTEN-Mediated Regulation of Apical Membrane Size.
Mol Cell Biol
; 38(12)2018 06 15.
Article
em En
| MEDLINE
| ID: mdl-29581186
ABSTRACT
PTEN is a tumor suppressor that is frequently lost in epithelial malignancies. A part of the tumor-suppressive properties of PTEN is attributed to its function in cell polarization and consequently its role in maintaining epithelial tissue integrity. However, surprisingly little is known about the function and regulation of PTEN during epithelial cell polarization. We used clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9-mediated gene disruption to delete PTEN in intestinal epithelial Ls174TW4 cells, which upon differentiation form a microvillus-covered apical membrane (brush border) on a part of the cell cortex, independent of cell-cell junctions. We show that loss of PTEN results in the formation of a larger brush border that, in a fraction of the cells, even spans the entire plasma membrane, revealing that PTEN functions in the regulation of apical membrane size. Depletion of the phosphatase PTPL1 resulted in a similar defect. PTPL1 interacts with PTEN, and this interaction is necessary for apical membrane enrichment of PTEN. Importantly, phosphatase activity of PTPL1 is not required, indicating that PTPL1 functions as an anchor protein in this process. Our work thus demonstrates a novel function for PTEN during cell polarization in controlling apical membrane size and identifies PTPL1 as a critical apical membrane anchor for PTEN in this process.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Membrana Celular
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Polaridade Celular
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PTEN Fosfo-Hidrolase
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Proteína Tirosina Fosfatase não Receptora Tipo 13
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Microvilosidades
Limite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Mol Cell Biol
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Holanda