N-(3-oxododecanoyl)-l-homoserine lactone modulates mitochondrial function and suppresses proliferation in intestinal goblet cells.
Life Sci
; 201: 81-88, 2018 May 15.
Article
em En
| MEDLINE
| ID: mdl-29596921
ABSTRACT
AIMS:
The quorum-sensing molecule N(3oxododecanoyl)lhomoserine lactone (C12-HSL), produced by the Gram negative human pathogenic bacterium Pseudomonas aeruginosa, modulates mammalian cell behavior. Our previous findings suggested that C12-HSL rapidly decreases viability and induces apoptosis in LS174T goblet cells. MAINMETHODS:
In this study, the effects of 100⯵M C12-HSL on mitochondrial function and cell proliferation in LS174T cells treated for 4â¯h were evaluated by real-time PCR, enzyme-linked immunosorbent assay (ELISA) and flow cytometry. KEYFINDINGS:
The results showed that the activities of mitochondrial respiratory chain complexes IV and V were significantly increased (Pâ¯<â¯0.05) in LS174T cells after C12-HSL treatment, with elevated intracellular ATP generation (Pâ¯<â¯0.05). Flow cytometry analysis revealed significantly increased intracellular Ca2+ levels (Pâ¯<â¯0.05), as well as disrupted mitochondrial activity and cell cycle arrest upon C12-HSL treatment. Apoptosis and cell proliferation related genes showed markedly altered expression levels (Pâ¯<â¯0.05) in LS174T cells after C12-HSL treatment. Moreover, the paraoxonase 2 (PON2) inhibitor TQ416 (1⯵M) remarkably reversed the above C12-HSL associated effects in LS174T cells.SIGNIFICANCE:
These findings indicated that C12-HSL alters mitochondrial energy production and function, and inhibits cell proliferation in LS174T cells, with PON2 involvement.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
4-Butirolactona
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Células Caliciformes
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Percepção de Quorum
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Homosserina
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Intestinos
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Mitocôndrias
Limite:
Humans
Idioma:
En
Revista:
Life Sci
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China