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An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein.
Dewari, Pooran Singh; Southgate, Benjamin; Mccarten, Katrina; Monogarov, German; O'Duibhir, Eoghan; Quinn, Niall; Tyrer, Ashley; Leitner, Marie-Christin; Plumb, Colin; Kalantzaki, Maria; Blin, Carla; Finch, Rebecca; Bressan, Raul Bardini; Morrison, Gillian; Jacobi, Ashley M; Behlke, Mark A; von Kriegsheim, Alex; Tomlinson, Simon; Krijgsveld, Jeroen; Pollard, Steven M.
Afiliação
  • Dewari PS; Edinburgh Cancer Research United Kingdom Centre, University of Edinburgh, Edinburgh, United Kingdom.
  • Southgate B; MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom.
  • Mccarten K; Edinburgh Cancer Research United Kingdom Centre, University of Edinburgh, Edinburgh, United Kingdom.
  • Monogarov G; MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom.
  • O'Duibhir E; Edinburgh Cancer Research United Kingdom Centre, University of Edinburgh, Edinburgh, United Kingdom.
  • Quinn N; MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom.
  • Tyrer A; German Cancer Research Center, University of Heidelberg, Heidelberg, Germany.
  • Leitner MC; Edinburgh Cancer Research United Kingdom Centre, University of Edinburgh, Edinburgh, United Kingdom.
  • Plumb C; MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom.
  • Kalantzaki M; Institute of Genetics and Molecular Medicine, Edinburgh Cancer Research United Kingdom Centre, University of Edinburgh, Edinburgh, United Kingdom.
  • Blin C; Edinburgh Cancer Research United Kingdom Centre, University of Edinburgh, Edinburgh, United Kingdom.
  • Finch R; MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom.
  • Bressan RB; Edinburgh Cancer Research United Kingdom Centre, University of Edinburgh, Edinburgh, United Kingdom.
  • Morrison G; MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom.
  • Jacobi AM; Edinburgh Cancer Research United Kingdom Centre, University of Edinburgh, Edinburgh, United Kingdom.
  • Behlke MA; MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom.
  • von Kriegsheim A; Edinburgh Cancer Research United Kingdom Centre, University of Edinburgh, Edinburgh, United Kingdom.
  • Tomlinson S; MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom.
  • Krijgsveld J; Edinburgh Cancer Research United Kingdom Centre, University of Edinburgh, Edinburgh, United Kingdom.
  • Pollard SM; MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom.
Elife ; 72018 04 11.
Article em En | MEDLINE | ID: mdl-29638216
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ribonucleoproteínas / Células-Tronco / Fatores de Transcrição / Mapeamento de Epitopos / Ensaios de Triagem em Larga Escala / Sistemas CRISPR-Cas / Proteína 9 Associada à CRISPR Limite: Animals / Humans Idioma: En Revista: Elife Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Reino Unido
Texto completo
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XML
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Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ribonucleoproteínas / Células-Tronco / Fatores de Transcrição / Mapeamento de Epitopos / Ensaios de Triagem em Larga Escala / Sistemas CRISPR-Cas / Proteína 9 Associada à CRISPR Limite: Animals / Humans Idioma: En Revista: Elife Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Reino Unido