Dengue virus infection induces chromatin remodeling at locus AAEL006536 in the midgut of Aedes aegypti.
Salud Publica Mex
; 60(1): 41-47, 2018.
Article
em En
| MEDLINE
| ID: mdl-29689655
ABSTRACT
OBJECTIVE:
To identify and characterize Aedes aegypti's AAEL006536 gene proximal upstream cis-regulatory sequences activated by dengue virus infection. MATERIALS ANDMETHODS:
A. aegypti Rockefeller strain mosquitoes were blood fed or infected with dengue virus 2. Open chromatinprofiling was then carried out in pools of midguts from each group of mosquitoes.RESULTS:
The proximal upstream region does not contain open chromatin sites in the midguts of blood-fed mosquitoes as detected by FAIRE-qPCR. In contrast, two cis-regulatory sites were identified in the same upstream region of dengue virus-infected mosquito midguts. The distal sequence contains STAT-, REL- and C/EBP-type transcription factor binding sites.CONCLUSIONS:
The activation of two proximal cis-regulatory sequences, induced by dengue virus infection, is mediated by chromatin remodeling mechanisms. Binding sites suggest a dengue virus infectioninduced participation of immunity transcription factors in the up-regulation of this gene. This suggests the participation of the AAEL006536 gene in the mosquito's antiviral innate immune response.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genes de Insetos
/
Proteínas de Insetos
/
Aedes
/
Vírus da Dengue
/
Mosquitos Vetores
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Salud Publica Mex
Ano de publicação:
2018
Tipo de documento:
Article