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Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing.
Xu, An; Zhou, Ruoji; Tu, Jian; Huo, Zijun; Zhu, Dandan; Wang, Donghui; Gingold, Julian A; Mata, Helen; Rao, Pulivarthi H; Liu, Mo; Mohamed, Alaa M T; Kong, Celine Shuet Lin; Jewell, Brittany E; Xia, Weiya; Zhao, Ruiying; Hung, Mien-Chie; Lee, Dung-Fang.
Afiliação
  • Xu A; Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
  • Zhou R; Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; The University of Texas, MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, TX 77030, USA.
  • Tu J; Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; Department of Musculoskeletal Oncology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, PR China.
  • Huo Z; Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; Department of Endocrinology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, PR China.
  • Zhu D; Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
  • Wang D; Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, PR China.
  • Gingold JA; Women's Health Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA.
  • Mata H; Department of Pediatrics, Baylor College of Medicine, Texas Children's Cancer and Hematology Centers, Houston, TX 77030, USA.
  • Rao PH; Department of Pediatrics, Baylor College of Medicine, Texas Children's Cancer and Hematology Centers, Houston, TX 77030, USA.
  • Liu M; Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
  • Mohamed AMT; Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
  • Kong CSL; Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
  • Jewell BE; Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; The University of Texas, MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, TX 77030, USA.
  • Xia W; Department of Molecular and Cellular Oncology, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA.
  • Zhao R; Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
  • Hung MC; Department of Molecular and Cellular Oncology, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA; Graduate Institute of Biomedical Sciences and Center for Molecular Medicine, China Medical University, Taichung 404, Taiwan.
  • Lee DF; Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; The University of Texas, MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, TX 77030, USA; Center for Stem C
Stem Cell Res ; 29: 215-219, 2018 05.
Article em En | MEDLINE | ID: mdl-29730572
ABSTRACT
Genetic mutations in TP53 contribute to multiple human cancers. Here we report the generation of a H1-p53(R248W/R248W) human embryonic stem cell line harboring a homozygous TP53 R248W mutation created by TALEN-mediated precise gene editing. The H1-p53(R248W/R248W) cell line maintains a normal karyotype, robust pluripotency gene expression, and the potential to differentiate to the three germ layers.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteína Supressora de Tumor p53 / Células-Tronco Embrionárias Humanas / Nucleases dos Efetores Semelhantes a Ativadores de Transcrição / Edição de Genes / Homozigoto / Mutação Limite: Humans / Male Idioma: En Revista: Stem Cell Res Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteína Supressora de Tumor p53 / Células-Tronco Embrionárias Humanas / Nucleases dos Efetores Semelhantes a Ativadores de Transcrição / Edição de Genes / Homozigoto / Mutação Limite: Humans / Male Idioma: En Revista: Stem Cell Res Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Estados Unidos