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The development of a novel AND logic based fluorescence probe for the detection of peroxynitrite and GSH.
Sedgwick, Adam C; Han, Hai-Hao; Gardiner, Jordan E; Bull, Steven D; He, Xiao-Peng; James, Tony D.
Afiliação
  • Sedgwick AC; Department of Chemistry , University of Bath , Bath , BA2 7AY , UK . Email: a.c.sedgwick@bath.ac.uk ; Email: s.d.bull@bath.ac.uk ; Email: t.d.james@bath.ac.uk.
  • Han HH; Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering , Feringa Nobel Prize Scientist Joint Research Center , School of Chemistry and Molecular Engineering , East China University of Science and Technology , 130 Meilong Rd.
  • Gardiner JE; Department of Chemistry , University of Bath , Bath , BA2 7AY , UK . Email: a.c.sedgwick@bath.ac.uk ; Email: s.d.bull@bath.ac.uk ; Email: t.d.james@bath.ac.uk.
  • Bull SD; Department of Chemistry , University of Bath , Bath , BA2 7AY , UK . Email: a.c.sedgwick@bath.ac.uk ; Email: s.d.bull@bath.ac.uk ; Email: t.d.james@bath.ac.uk.
  • He XP; Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering , Feringa Nobel Prize Scientist Joint Research Center , School of Chemistry and Molecular Engineering , East China University of Science and Technology , 130 Meilong Rd.
  • James TD; Department of Chemistry , University of Bath , Bath , BA2 7AY , UK . Email: a.c.sedgwick@bath.ac.uk ; Email: s.d.bull@bath.ac.uk ; Email: t.d.james@bath.ac.uk.
Chem Sci ; 9(15): 3672-3676, 2018 Apr 21.
Article em En | MEDLINE | ID: mdl-29780497
We have developed a novel AND logic based fluorescence probe for the simultaneous detection of ONOO- and GSH (GSH-PF3). The GSH-PF3 probe was synthesised over three steps starting from commercially available fluorescein. The probe was constructed by attaching the GSH reactive motif, 2,4-dinitrobenzenesulfonyl, to the previously reported boronate fluorescence based probe, PF3. GSH-PF3 produced only a small fluorescence response towards the addition of GSH or ONOO- separately. However, when the probe was exposed to both analytes, there was a significant (40-fold) fluorescence enhancement. GSH-PF3 demonstrated an excellent selectivity towards both GSH and ONOO-. In cellular imaging experiments the probe was shown to be cell permeable with no 'turn-on' response observed for the addition of either GSH or ONOO- separately. However, in the presence of both analytes, a clear fluorescence response was observed in live cells. GSH-PF3 was further able to monitor the co-existence of metabolically produced ONOO- and GSH by exogenous stimulation.

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies Idioma: En Revista: Chem Sci Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies Idioma: En Revista: Chem Sci Ano de publicação: 2018 Tipo de documento: Article