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TNF-α induction of IL-6 in alveolar type II epithelial cells: Contributions of JNK/c-Jun/AP-1 element, C/EBPδ/C/EBP binding site and IKK/NF-κB p65/κB site.
Yan, Chunguang; Deng, Chunmin; Liu, Xiufang; Chen, Yutong; Ye, Jiawei; Cai, Rentian; Shen, Yanfei; Tang, Huifang.
Afiliação
  • Yan C; Department of Pathogenic Biology and Immunology, Medical School of Southeast University, Nanjing, 210009, China. Electronic address: ycgagcy@163.com.
  • Deng C; Department of Pathogenic Biology and Immunology, Medical School of Southeast University, Nanjing, 210009, China. Electronic address: 344260738@qq.com.
  • Liu X; Department of Pathogenic Biology and Immunology, Medical School of Southeast University, Nanjing, 210009, China.
  • Chen Y; Department of Pathogenic Biology and Immunology, Medical School of Southeast University, Nanjing, 210009, China.
  • Ye J; Department of Pathogenic Biology and Immunology, Medical School of Southeast University, Nanjing, 210009, China.
  • Cai R; Department of Infectious Diseases, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, China.
  • Shen Y; Department of Bioengineering, Medical School of Southeast University, Nanjing, 210009, China.
  • Tang H; Zhejiang Respiratory Drugs Research Laboratory of the State Food and Drug Administration of China, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, China. Electronic address: tanghuifang@zju.edu.cn.
Mol Immunol ; 101: 585-596, 2018 09.
Article em En | MEDLINE | ID: mdl-29887504
Although participation of IL-6 in lung inflammation has been widely elucidated, the transcriptional regulation of its generation in alveolar type II cells stimulated by TNF-α remain unclear. Here, we find that TNF-α significantly induces IL-6 production, and TNF-α induction of IL-6 is mainly regulated at transcriptional level. Upon stimulated by TNF-α, Activator Protein-1 (AP-1)-mediated transcriptional activity is apparently increased in alveolar type II epithelial cells, which might be derived from elevated phosphorylation of JNK and subsequent activation of c-Jun. Either down-regulation of c-Jun or the AP-1 site mutation leads to significant reduction of IL-6 expression. In contrast, ectopic expression of c-Jun notably increases IL-6 generation. So, c-Jun, one of the AP-1 family members, plays a pivotal role in TNF-α-induced IL-6 generation. CCAAT/enhancer binding protein δ (C/EBPδ) expression is significantly amplified by TNF-α, which may contribute to the rise of C/EBP activity in alveolar type II cells. C/EBPδ shRNA treatment results in attenuation of IL-6 expression in the cells, which is consistent with data by introduction of mutations into the C/EBP site in the promoter. However, overexpression of C/EBPδ greatly increases the IL-6 promoter activity. In addition, data regarding another transactivator in the family-C/EBPß show that it does not affect IL-6 production. We also find that the IKK/NF-κB p65 pathway is activated in TNF-α-treated alveolar type II epithelial cells, and plays an essential role in positive regulation of IL-6 expression in TNF-α-treated alveolar type II epithelial cells via knockdown or forced expression of NF-κB p65, or elimination of κB sites in the IL-6 promoter. Notably, IL-6 promoter-driven luciferase production in primary alveolar type II epithelial cells can also be increased by the ectopic expression of c-Jun, C/EBPδ, and NF-κB p65, respectively. Collectively, our data provide insights into molecular mechanism involved in IL-6 expression in alveolar type II epithelial cells on TNF-α treatment, which provides a theoretical basis for specific inhibition of IL-6 production at the transcriptional level.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Interleucina-6 / Fator de Necrose Tumoral alfa / Proteínas Proto-Oncogênicas c-jun / Fator de Transcrição AP-1 / Proteínas Estimuladoras de Ligação a CCAAT / Proteínas Quinases JNK Ativadas por Mitógeno / Quinase I-kappa B / Fator de Transcrição RelA / Células Epiteliais Alveolares Limite: Animals Idioma: En Revista: Mol Immunol Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Interleucina-6 / Fator de Necrose Tumoral alfa / Proteínas Proto-Oncogênicas c-jun / Fator de Transcrição AP-1 / Proteínas Estimuladoras de Ligação a CCAAT / Proteínas Quinases JNK Ativadas por Mitógeno / Quinase I-kappa B / Fator de Transcrição RelA / Células Epiteliais Alveolares Limite: Animals Idioma: En Revista: Mol Immunol Ano de publicação: 2018 Tipo de documento: Article