Inhibition of HIV-1 infection by human pegivirus type 1-derived peptides is affected by human pegivirus type 1 genotype and HIV-1 coreceptor tropism.
AIDS
; 32(14): 1951-1957, 2018 09 10.
Article
em En
| MEDLINE
| ID: mdl-29912064
ABSTRACT
OBJECTIVE(S) Up to 40% of HIV-1 infected individuals are coinfected with human pegivirus type 1 (HPgV-1). The majority of studies, but not all, have reported a beneficial effect of HPgV-1 coinfection on HIV-1 disease progression. So far, the impact of different HPgV-1 genotypes on different HIV-1 subtypes remains unclear. METHODS:
Peptides derived from HPgV-1 envelope protein E2, and representing different viral genotypes, were synthesized using Fmoc/t-Bu-based solid phase peptide synthesis. The inhibitory effect of these peptides on the infection of reporter cell lines was tested using an HIV-1 subtype panel representing clades A (nâ=â2), AG (nâ=â2), B (nâ=â6), C (nâ=â2), D (nâ=â2), F (nâ=â2), G (nâ=â1), G/H (nâ=â1), and group O (nâ=â2).RESULTS:
HIV-1 infection was blocked more efficiently by peptides derived from HPgV-1 GT2 than GT1 (Pâ=â0.05). The HIV-1 subtype did not affect the degree of inhibition by a peptide derived from HPgV-1 GT2. All CXCR4-/dual-tropic isolates (nâ=â12), but only half (four out of eight) CCR5-tropic viruses were inhibited by this peptide (Pâ=â0.014).CONCLUSION:
Our data indicate that the inhibitory effect of peptides derived from HPgV-1 E2 protein is dependent on the genotype, suggesting that coinfection with HPgV-1 GT1 is less likely to confer a beneficial effect on HIV-1 disease progression than GT2. The preferential suppression of more pathogenic CXCR4-tropic HIV-1 by peptides derived from HPgV-1 GT2 may explain the favorable effect in patients harboring these HIV-1 isolates. Consequently, HPgV-1 genotype and HIV-1 coreceptor tropism are likely determinants for the beneficial effect of HPgV-1 co-infection in HIV-1-infected individuals.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Interferência Viral
/
HIV-1
/
Flaviviridae
/
Internalização do Vírus
/
Tropismo Viral
Limite:
Humans
Idioma:
En
Revista:
AIDS
Assunto da revista:
SINDROME DA IMUNODEFICIENCIA ADQUIRIDA (AIDS)
Ano de publicação:
2018
Tipo de documento:
Article