Improving the accuracy of pancreatic cancer clinical staging by exploitation of nanoparticle-blood interactions: A pilot study.
Pancreatology
; 18(6): 661-665, 2018 Sep.
Article
em En
| MEDLINE
| ID: mdl-29914752
ABSTRACT
BACKGROUND:
Pancreatic ductal adenocarcinoma (PDAC) early diagnosis is⯠crucial â¯and new, cheap and user-friendly techniques for biomarker identification⯠are â¯needed. "Protein corona" (PC) is emerging a new bio-interface potentially useful in tumor early diagnosis. In a previous investigation, we showed that relevant differences between the â¯protein patterns of⯠PCs formed on lipid NPs after exposure to PDAC and non-cancer plasma⯠samples exist. To extend that research, We performed this pilot study to investigate the effect of PDAC tumor size and distant metastases on PC composition.METHODS:
Twenty PDACs were clinically staged according to the UICC TNM staging system 8â¯tâ¯hâ¯Edition. Collected plasma samples were let to interact with lipid NPs; resulting PCs were characterized by SDS-PAGE. To properly evaluate changes in the PC, the protein intensity profiles were reduced to four regions of molecular weight < 25â¯kDa, 25-50â¯kDa, 50-120â¯kDa, > 120â¯kDa.â¯RESULTS:
Data analysis allowed toâ¯distinguish T1-T2 cases from T3 andâ¯above all fromâ¯metastatic ones (pâ¯<â¯0.05). Discrimination power was particularly due to a subset of plasma proteins with molecular â¯weight comprised between 25-50â¯kDa â¯and 50-120â¯kDa.CONCLUSIONS:
PC composition is critically influenced by tumor size and presence of distant metastases in PDAC. If our findings will be further confirmed, we envision that future developments of cheap and user-friendly PC-based tools will allow to improve the accuracy of PDAC clinical staging, identifying among resectable â¯PDACs with potentially better prognosis (i.e. T1 and T2) thoseâ¯at higher risk of occult distant metastases.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Pancreáticas
/
Carcinoma Ductal Pancreático
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Nanopartículas
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Lipossomos
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Limite:
Aged
/
Aged80
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Female
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Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Pancreatology
Assunto da revista:
ENDOCRINOLOGIA
/
GASTROENTEROLOGIA
Ano de publicação:
2018
Tipo de documento:
Article