Assignment of ESR signals of Escherichia coli terminal oxidase complexes.
Biochim Biophys Acta
; 810(1): 62-72, 1985 Oct 29.
Article
em En
| MEDLINE
| ID: mdl-2994724
ABSTRACT
The ESR signals of all the major components of the aerobic respiratory chain of Escherichia coli were measured and assigned at liquid helium temperature. Cytochrome b-556 gives a weak high-spin signal at g = 6.0. The terminal oxidase cytochrome b-562 . o complex gives signals at g = 6.0, 3.0 and 2.26, and the terminal oxidase cytochrome b-558 . d complex gives signals at g = 6.0, 2.5 and 2.3. A signal derived from cupric ions in the purified cytochrome b-562 . o complex was observed near g = 2.0. It was shown by the effects of KCN or NaN3 on cytochromes under the air-oxidized conditions that cytochrome o has a high-spin heme and cytochrome d has a low-spin heme. The E'm values for cytochromes b-558 and d, respectively, determined by potentiometric titration of the ESR signals were 140 and 240 mV in the membrane preparation, and 30 and 240 mV in the purified preparation. The oxidized cytochrome d gave intense low-spin signals at g = 2.5 and 2.3, while cytochrome d under the air-oxidized conditions gave corresponding signals of only very low intensity. These results suggested that most of the cytochrome d under the air-oxidized conditions contains a diamagnetic iron atom with a bound dioxygen.
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Base de dados:
MEDLINE
Assunto principal:
Espectroscopia de Ressonância de Spin Eletrônica
/
NADPH Oxidases
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Citocromos
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Proteínas de Escherichia coli
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Escherichia coli
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1985
Tipo de documento:
Article