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Genome-Wide Sequence Analysis of Kaposi Sarcoma-Associated Herpesvirus Shows Diversification Driven by Recombination.
Sallah, Neneh; Palser, Anne L; Watson, Simon J; Labo, Nazzarena; Asiki, Gershim; Marshall, Vickie; Newton, Robert; Whitby, Denise; Kellam, Paul; Barroso, Inês.
Afiliação
  • Sallah N; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge.
  • Palser AL; Kymab Ltd, Babraham Research Complex, Cambridge.
  • Watson SJ; Kymab Ltd, Babraham Research Complex, Cambridge.
  • Labo N; AIDS and Cancer Virus Program, Viral Oncology Section, Leidos Biomedical Research, Frederick National Laboratory for Cancer Research, Maryland.
  • Asiki G; Medical Research Council/Uganda Virus Research Institute, Uganda Research Unit, London School of Hygiene and Tropical Medicine, United Kingdom.
  • Marshall V; AIDS and Cancer Virus Program, Viral Oncology Section, Leidos Biomedical Research, Frederick National Laboratory for Cancer Research, Maryland.
  • Newton R; Medical Research Council/Uganda Virus Research Institute, Uganda Research Unit, London School of Hygiene and Tropical Medicine, United Kingdom.
  • Whitby D; AIDS and Cancer Virus Program, Viral Oncology Section, Leidos Biomedical Research, Frederick National Laboratory for Cancer Research, Maryland.
  • Kellam P; Kymab Ltd, Babraham Research Complex, Cambridge.
  • Barroso I; Department of Medicine, Imperial College London, United Kingdom.
J Infect Dis ; 218(11): 1700-1710, 2018 10 20.
Article em En | MEDLINE | ID: mdl-30010810
ABSTRACT

Background:

Kaposi sarcoma-associated herpesvirus (KSHV) establishes lifelong infection in the human host and has been associated with a variety of malignancies. KSHV displays striking geographic variation in prevalence, which is highest in sub-Saharan Africa. The current KSHV genome sequences available are all tumor cell line-derived or primary tumor-associated viruses, which have provided valuable insights into KSHV genetic diversity.

Methods:

Here, we sequenced 45 KSHV genomes from a Ugandan population cohort in which KSHV is endemic; these are the only genome sequences obtained from nondiseased individuals and of KSHV DNA isolated from saliva.

Results:

Population structure analysis, along with the 25 published genome sequences from other parts of the world, showed whole-genome variation, separating sequences and variation within the central genome contributing to clustering of genomes by geography. We reveal new evidence for the presence of intragenic recombination and multiple recombination events contributing to the divergence of genomes into at least 5 distinct types.

Discussion:

This study shows that large-scale genome-wide sequencing from clinical and epidemiological samples is necessary to capture the full extent of genetic diversity of KSHV, including recombination, and provides evidence to suggest a revision of KSHV genotype nomenclature.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Recombinação Genética / Sarcoma de Kaposi / Herpesvirus Humano 8 / Genômica Tipo de estudo: Etiology_studies / Incidence_studies / Observational_studies / Risk_factors_studies Limite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged País/Região como assunto: Africa Idioma: En Revista: J Infect Dis Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Recombinação Genética / Sarcoma de Kaposi / Herpesvirus Humano 8 / Genômica Tipo de estudo: Etiology_studies / Incidence_studies / Observational_studies / Risk_factors_studies Limite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged País/Região como assunto: Africa Idioma: En Revista: J Infect Dis Ano de publicação: 2018 Tipo de documento: Article