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Hepatitis C virus enters liver cells using the CD81 receptor complex proteins calpain-5 and CBLB.
Bruening, Janina; Lasswitz, Lisa; Banse, Pia; Kahl, Sina; Marinach, Carine; Vondran, Florian W; Kaderali, Lars; Silvie, Olivier; Pietschmann, Thomas; Meissner, Felix; Gerold, Gisa.
Afiliação
  • Bruening J; Insitute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Medical School Hannover and the Helmholtz Centre for Infection Research, Hannover, Germany.
  • Lasswitz L; Insitute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Medical School Hannover and the Helmholtz Centre for Infection Research, Hannover, Germany.
  • Banse P; Insitute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Medical School Hannover and the Helmholtz Centre for Infection Research, Hannover, Germany.
  • Kahl S; Insitute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Medical School Hannover and the Helmholtz Centre for Infection Research, Hannover, Germany.
  • Marinach C; Sorbonne Université, INSERM, CNRS, Centre d'Immunologie et des Maladies Infectieuses, CIMI-Paris, Paris, France.
  • Vondran FW; Department of General, Visceral and Transplant Surgery, Hannover Medical School, Hannover, Germany.
  • Kaderali L; Institute of Bioinformatics, University Medicine Greifswald, Greifswald, Germany.
  • Silvie O; Sorbonne Université, INSERM, CNRS, Centre d'Immunologie et des Maladies Infectieuses, CIMI-Paris, Paris, France.
  • Pietschmann T; Insitute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Medical School Hannover and the Helmholtz Centre for Infection Research, Hannover, Germany.
  • Meissner F; Department of Experimental Systems Immunology, Max Planck Institute of Biochemistry, Martinsried, Germany.
  • Gerold G; Insitute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Medical School Hannover and the Helmholtz Centre for Infection Research, Hannover, Germany.
PLoS Pathog ; 14(7): e1007111, 2018 07.
Article em En | MEDLINE | ID: mdl-30024968
Hepatitis C virus (HCV) and the malaria parasite Plasmodium use the membrane protein CD81 to invade human liver cells. Here we mapped 33 host protein interactions of CD81 in primary human liver and hepatoma cells using high-resolution quantitative proteomics. In the CD81 protein network, we identified five proteins which are HCV entry factors or facilitators including epidermal growth factor receptor (EGFR). Notably, we discovered calpain-5 (CAPN5) and the ubiquitin ligase Casitas B-lineage lymphoma proto-oncogene B (CBLB) to form a complex with CD81 and support HCV entry. CAPN5 and CBLB were required for a post-binding and pre-replication step in the HCV life cycle. Knockout of CAPN5 and CBLB reduced susceptibility to all tested HCV genotypes, but not to other enveloped viruses such as vesicular stomatitis virus and human coronavirus. Furthermore, Plasmodium sporozoites relied on a distinct set of CD81 interaction partners for liver cell entry. Our findings reveal a comprehensive CD81 network in human liver cells and show that HCV and Plasmodium highjack selective CD81 interactions, including CAPN5 and CBLB for HCV, to invade cells.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Calpaína / Hepacivirus / Proteínas Adaptadoras de Transdução de Sinal / Proteínas Proto-Oncogênicas c-cbl / Internalização do Vírus / Interações Hospedeiro-Patógeno / Tetraspanina 28 Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: PLoS Pathog Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Calpaína / Hepacivirus / Proteínas Adaptadoras de Transdução de Sinal / Proteínas Proto-Oncogênicas c-cbl / Internalização do Vírus / Interações Hospedeiro-Patógeno / Tetraspanina 28 Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: PLoS Pathog Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Alemanha