Overexpression and purification of human myosins from transiently and stably transfected suspension adapted HEK293SF-3F6 cells.

Usaj, Marko; Zattelman, Lilach; Regev, Ronit; Shneyer, Boris I; Wiesel-Motiuk, Naama; Henn, Arnon

Usaj, Marko; Zattelman, Lilach; Regev, Ronit; Shneyer, Boris I; Wiesel-Motiuk, Naama; Henn, Arnon.

Afiliação

Usaj M; Faculty of Biology, Technion- Israel Institute of Technology, Haifa, 3200003, Israel.
Zattelman L; Faculty of Biology, Technion- Israel Institute of Technology, Haifa, 3200003, Israel; Russell Berrie Nanotechnology Institute, Technion-Israel Institute of Technology, Haifa 3200003, Israel.
Regev R; Faculty of Biology, Technion- Israel Institute of Technology, Haifa, 3200003, Israel.
Shneyer BI; Faculty of Biology, Technion- Israel Institute of Technology, Haifa, 3200003, Israel.
Wiesel-Motiuk N; Faculty of Biology, Technion- Israel Institute of Technology, Haifa, 3200003, Israel.
Henn A; Faculty of Biology, Technion- Israel Institute of Technology, Haifa, 3200003, Israel. Electronic address: arnon.henn@technion.ac.il.

Anal Biochem ; 558: 19-27, 2018 10 01.

Article em En | MEDLINE | ID: mdl-30075102

Assuntos

Miosinas/isolamento & purificação; Miosinas/metabolismo; Animais; Técnicas de Cultura de Células; Dictyostelium/metabolismo; Células HEK293; Humanos; Camundongos; Miosinas/genética; Regiões Promotoras Genéticas; Transfecção

Palavras-chave

Gene expression; HEK293SF-3F6; Human cell expression system; Polyethyleneimine; Recombinant myosins; Suspension cell culture

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Miosinas Tipo de estudo: Guideline Limite: Animals / Humans Idioma: En Revista: Anal Biochem Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Israel

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