Inhibition of lysyl oxidase expression by dextran sulfate affects invasion and migration of gastric cancer cells.
Int J Mol Med
; 42(5): 2737-2749, 2018 Nov.
Article
em En
| MEDLINE
| ID: mdl-30226558
ABSTRACT
In the present study, the effect of dextran sulfate (DS) on the metastasis and invasion of human gastric cancer cells and its key underlying mechanism were investigated. The levels of hypoxiainducible factor 1α (HIF1α), transforming growth factor ß (TGFß) and lysyl oxidase (LOX) expression were evaluated in human gastric cancer and peritumoral tissues by immunohistochemical analysis. Cell proliferation and apoptosis were also examined using the Cell Counting Kit8 assay and flow cytometry. The effect of DS on the invasion and migration of BGC823 cells was assessed using a Transwell assay. BGC823 cells were divided into the control (phosphatebuffered salinetreated) and experimental (DStreated) groups, and cultured for different times under hypoxic conditions. Subsequently, LOX and TGFß expression levels in the cells were measured by immunocytochemistry, immunofluorescence, reverse transcriptionquantitative polymerase chain reaction and western blot analysis. HIF1α, TGFß and LOX expression levels were significantly higher in human gastric cancer tissues as compared with that in adjacent tissues. DS influenced cell proliferation and apoptosis in a dosedependent manner. Furthermore, DS reduced the number of invaded and migrated cells. Under hypoxic conditions, DS reduced HIF1α, TGFß and LOX expression levels in BGC823 cells. After 12 h, the effect of combination of DS and ßaminopropionitrile (BAPN) on LOX and TGFß protein levels was more significant compared with that of DS or BAPN alone. Therefore, DS may inhibit the invasion and migration of human gastric cancer cells under hypoxic conditions by influencing LOX.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Gástricas
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Regulação para Baixo
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Sulfato de Dextrana
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Proteína-Lisina 6-Oxidase
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Invasividade Neoplásica
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Antineoplásicos
Limite:
Humans
Idioma:
En
Revista:
Int J Mol Med
Assunto da revista:
BIOLOGIA MOLECULAR
/
GENETICA MEDICA
Ano de publicação:
2018
Tipo de documento:
Article