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Characterization of an antigenic serine protease in the Trichinella spiralis adult.
Gao, He; Tang, Bin; Bai, Xue; Wang, Libo; Wu, Xiuping; Shi, Haining; Wang, Xuelin; Liu, Xiaolei; Liu, Mingyuan.
Afiliação
  • Gao H; Key Lab for Zoonoses Research, Ministry of Education, Institute of Zoonoses, Jilin University, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, PR China.
  • Tang B; Key Lab for Zoonoses Research, Ministry of Education, Institute of Zoonoses, Jilin University, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, PR China.
  • Bai X; Key Lab for Zoonoses Research, Ministry of Education, Institute of Zoonoses, Jilin University, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, PR China.
  • Wang L; Key Lab for Zoonoses Research, Ministry of Education, Institute of Zoonoses, Jilin University, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, PR China.
  • Wu X; Key Lab for Zoonoses Research, Ministry of Education, Institute of Zoonoses, Jilin University, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, PR China.
  • Shi H; Mucosal Immunology Laboratory, Pediatric Gastroenterology Unit, Massachusetts General Hospital East, USA.
  • Wang X; Key Lab for Zoonoses Research, Ministry of Education, Institute of Zoonoses, Jilin University, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, PR China.
  • Liu X; Key Lab for Zoonoses Research, Ministry of Education, Institute of Zoonoses, Jilin University, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, PR China. Electronic address: liuxlei@163.com.
  • Liu M; Key Lab for Zoonoses Research, Ministry of Education, Institute of Zoonoses, Jilin University, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zo
Exp Parasitol ; 195: 8-18, 2018 Dec.
Article em En | MEDLINE | ID: mdl-30248330
ABSTRACT
Serine proteases have been identified as important molecules that are involved in many parasitic infections, and these molecules have also been suggested to play important roles in Trichinella spiralis infections. In the present study, the antigenic serine protease gene Ts-ADSp-7, which was screened from a cDNA library of Trichinella spiralis Adults at 3 days post-infection (p.i.), was cloned and expressed in Escherichia coli. The encoded protein, Ts-ADSp-7, revealed a potential trypsin-like serine protease domain but lacked substrate banding site at position 227 and protease activity. Transcription could be detected in the Adult and muscle larval stage but not in the newborn larval stage, where no fluorescent signal was detected. Western blot analysis revealed that the 3 days p.i. Adults and muscle larvae could secrete Ts-ADSp-7. Interestingly, strong fluorescent signal of Ts-ADSp-7 could be detected in the nucleoli of the enlarged muscle cell nuclei from 12 to 16 days p.i. and in the ß-stichosomes of the muscle larvae from 16 to 35 days p.i.. The coagulation assay indicated that Ts-ADSp-7 could inhibit intrinsic coagulation pathway. Regarding the putatively important function of the serine protease in the helminth infection to hosts, a total of 81 serine proteases were found in the parasite and mainly comprised eight subfamilies. These subfamilies exhibited high similarity to transmembrane serine protease, coagulation factor XI, lipocalin, guanylin, ceropin, kallikrein, and plasminogen. Moreover, stage specificity was detected in several subfamilies. In summary, the putatively inactive serine protease-like protein Ts-ADSp-7 could inhibit blood coagulation, and the protein is located in the enlarged nuclei of nurse cells during capsule formation. Furthermore, members of the serine protease family in the parasite might be important molecules in the parasite-host interaction.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Trichinella spiralis / Serina Proteases / Antígenos de Helmintos Tipo de estudo: Prognostic_studies Idioma: En Revista: Exp Parasitol Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Trichinella spiralis / Serina Proteases / Antígenos de Helmintos Tipo de estudo: Prognostic_studies Idioma: En Revista: Exp Parasitol Ano de publicação: 2018 Tipo de documento: Article