Ultraviolet C light efficiently inactivates nonenveloped hepatitis A virus and feline calicivirus in platelet concentrates.
Transfusion
; 58(11): 2669-2674, 2018 11.
Article
em En
| MEDLINE
| ID: mdl-30267410
ABSTRACT
BACKGROUND:
Nonenveloped transfusion-transmissible viruses such as hepatitis A virus (HAV) and hepatitis E virus (HEV) are resistant to many of the common virus inactivation procedures for blood products. This study investigated the pathogen inactivation (PI) efficacy of the THERAFLEX UV-Platelets system against two nonenveloped viruses HAV and feline calicivirus (FCV), in platelet concentrates (PCs). STUDY DESIGN ANDMETHODS:
PCs in additive solution were spiked with high titers of cell culture-derived HAV and FCV, and treated with ultraviolet C at various doses. Pre- and posttreatment samples were taken and the level of viral infectivity determined at each dose. For some samples, large-volume plating was performed to improve the detection limit of the virus assay.RESULTS:
THERAFLEX UV-Platelets reduced HAV titers in PCs to the limit of detection, resulting in a virus reduction factor of greater than 4.2 log steps, and reduced FCV infectivity in PCs by 3.0 ± 0.2 log steps.CONCLUSIONS:
THERAFLEX UV-Platelets effectively inactivates HAV and FCV in platelet units.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Raios Ultravioleta
/
Plaquetas
/
Calicivirus Felino
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Vírus da Hepatite A
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Transfusion
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Alemanha