Introducing nano-scale quantitative polymerase chain reaction.

Dyrstad, Sissel E; Tusubira, Deusdedit; Knappskog, Stian; Tronstad, Karl J; Røsland, Gro V

Dyrstad, Sissel E; Tusubira, Deusdedit; Knappskog, Stian; Tronstad, Karl J; Røsland, Gro V.

Afiliação

Dyrstad SE; Department of Biomedicine, University of Bergen, Norway.
Tusubira D; Department of Biomedicine, University of Bergen, Norway.
Knappskog S; Department of Clinical Science, University of Bergen, Bergen, Norway; Department of Oncology, Haukeland University Hospital, Bergen, Norway.
Tronstad KJ; Department of Biomedicine, University of Bergen, Norway.
Røsland GV; Department of Biomedicine, University of Bergen, Norway; Department of Oncology, Haukeland University Hospital, Bergen, Norway. Electronic address: gro.rosland@uib.no.

Biochem Biophys Res Commun ; 506(4): 923-926, 2018 12 02.

Article em En | MEDLINE | ID: mdl-30392909

ABSTRACT

ABSTRACT

We show that the quantitative PCR reaction volume can be reduced significantly compared to the standard procedures, without compromising data quality. By analyzing dilution series (100-10-7) we found that measurement in 1â¯µl reaction volume indeed gave valid results comparable to 10 µL, when using a routine pipetting robot. This may enable a significant cost reduction through cutback of both biological material as well as chemical reagents.

Assuntos

Nanotecnologia/métodos; Reação em Cadeia da Polimerase em Tempo Real/métodos; Linhagem Celular Tumoral; Humanos; Padrões de Referência

Palavras-chave

Nano-scale qPCR; TaqMan technology; qPCR

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nanotecnologia / Reação em Cadeia da Polimerase em Tempo Real Limite: Humans Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Noruega

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