Generation of Stable Expression Mammalian Cell Lines Using Lentivirus.
Bio Protoc
; 8(21)2018 Nov 05.
Article
em En
| MEDLINE
| ID: mdl-30505888
ABSTRACT
Lentiviruses are used very widely to generate stable expression mammalian cell lines. They are used for both gene down-regulation (by using shRNA) or for gene up-regulation (by using ORF of gene of interest). The technique of generating stable cell lines using 3rd generation lentivirus is very robust and it typically takes about 1-2 weeks to get stable expression for most mammalian cell lines. The advantage of using the 3rd generation lentivirus are that are very safe and they are replication incompetent.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Bio Protoc
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Estados Unidos