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Single tube liquid biopsy for advanced non-small cell lung cancer.
de Wit, Sanne; Rossi, Elisabetta; Weber, Sabrina; Tamminga, Menno; Manicone, Mariangela; Swennenhuis, Joost F; Groothuis-Oudshoorn, Catharina G M; Vidotto, Riccardo; Facchinetti, Antonella; Zeune, Leonie L; Schuuring, Ed; Zamarchi, Rita; Hiltermann, T Jeroen N; Speicher, Michael R; Heitzer, Ellen; Terstappen, Leon W M M; Groen, Harry J M.
Afiliação
  • de Wit S; Department of Medical Cell BioPhysics, University of Twente, Enschede, The Netherlands.
  • Rossi E; DISCOG, University of Padua, Padua, Italy.
  • Weber S; Veneto Institute of Oncology IOV - IRCCS, Padua, Italy.
  • Tamminga M; Institute of Human Genetics, Diagnostic and Research Centre for Molecular BioMedicine, Medical University of Graz, Graz, Austria.
  • Manicone M; Department of Pulmonary Diseases, University of Groningen, University Medical Centre Groningen, Groningen, The Netherlands.
  • Swennenhuis JF; Veneto Institute of Oncology IOV - IRCCS, Padua, Italy.
  • Groothuis-Oudshoorn CGM; Department of Medical Cell BioPhysics, University of Twente, Enschede, The Netherlands.
  • Vidotto R; Department of Health Technology & Services Research, University of Twente, Enschede, The Netherlands.
  • Facchinetti A; Veneto Institute of Oncology IOV - IRCCS, Padua, Italy.
  • Zeune LL; DISCOG, University of Padua, Padua, Italy.
  • Schuuring E; Veneto Institute of Oncology IOV - IRCCS, Padua, Italy.
  • Zamarchi R; Department of Medical Cell BioPhysics, University of Twente, Enschede, The Netherlands.
  • Hiltermann TJN; Department of Applied Mathematics, University of Twente, Enschede, The Netherlands.
  • Speicher MR; Department of Pulmonary Diseases, University of Groningen, University Medical Centre Groningen, Groningen, The Netherlands.
  • Heitzer E; Veneto Institute of Oncology IOV - IRCCS, Padua, Italy.
  • Terstappen LWMM; Veneto Institute of Oncology IOV - IRCCS, Padua, Italy.
  • Groen HJM; Institute of Human Genetics, Diagnostic and Research Centre for Molecular BioMedicine, Medical University of Graz, Graz, Austria.
Int J Cancer ; 144(12): 3127-3137, 2019 06 15.
Article em En | MEDLINE | ID: mdl-30536653
ABSTRACT
The need for a liquid biopsy in non-small cell lung cancer (NSCLC) patients is rapidly increasing. We studied the relation between overall survival (OS) and the presence of four cancer biomarkers from a single blood draw in advanced NSCLC patients EpCAMhigh circulating tumor cells (CTC), EpCAMlow CTC, tumor-derived extracellular vesicles (tdEV) and cell-free circulating tumor DNA (ctDNA). EpCAMhigh CTC were detected with CellSearch, tdEV in the CellSearch images and EpCAMlow CTC with filtration after CellSearch. ctDNA was isolated from plasma and mutations present in the primary tumor were tracked with deep sequencing methods. In 97 patients, 21% had ≥2 EpCAMhigh CTC, 15% had ≥2 EpCAMlow CTC, 27% had ≥18 tdEV and 19% had ctDNA with ≥10% mutant allele frequency. Either one of these four biomarkers could be detected in 45% of the patients and all biomarkers were present in 2%. In 11 out of 16 patients (69%) mutations were detected in the ctDNA. Two or more unfavorable biomarkers were associated with poor OS. The presence of EpCAMhigh CTC and elevated levels of tdEV and ctDNA was associated with a poor OS; however, the presence of EpCAMlow CTC was not. This single tube approach enables simultaneous analysis of multiple biomarkers to explore their potential as a liquid biopsy.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carcinoma Pulmonar de Células não Pequenas / Biópsia Líquida / Neoplasias Pulmonares Tipo de estudo: Clinical_trials / Observational_studies / Risk_factors_studies Limite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Int J Cancer Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carcinoma Pulmonar de Células não Pequenas / Biópsia Líquida / Neoplasias Pulmonares Tipo de estudo: Clinical_trials / Observational_studies / Risk_factors_studies Limite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Int J Cancer Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Holanda