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Murine hematopoietic stem cell activity is derived from pre-circulation embryos but not yolk sacs.
Ganuza, Miguel; Chabot, Ashley; Tang, Xing; Bi, Wenjian; Natarajan, Sivaraman; Carter, Robert; Gawad, Charles; Kang, Guolian; Cheng, Yong; McKinney-Freeman, Shannon.
Afiliação
  • Ganuza M; Department of Hematology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.
  • Chabot A; Department of Hematology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.
  • Tang X; Department of Hematology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.
  • Bi W; Department of Biostatistics, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.
  • Natarajan S; Department of Oncology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.
  • Carter R; Department of Oncology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.
  • Gawad C; Department of Oncology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.
  • Kang G; Department of Biostatistics, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.
  • Cheng Y; Department of Hematology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.
  • McKinney-Freeman S; Department of Hematology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA. Shannon.McKinney-Freeman@stjude.org.
Nat Commun ; 9(1): 5405, 2018 12 20.
Article em En | MEDLINE | ID: mdl-30573729
ABSTRACT
The embryonic site of definitive hematopoietic stem cell (dHSC) origination has been debated for decades. Although an intra-embryonic origin is well supported, the yolk sac (YS) contribution to adult hematopoiesis remains controversial. The same developmental origin makes it difficult to identify specific markers that discern between an intraembryonic versus YS-origin using a lineage trace approach. Additionally, the highly migratory nature of blood cells and the inability of pre-circulatory embryonic cells (i.e., 5-7 somite pairs (sp)) to robustly engraft in transplantation, even after culture, has precluded scientists from properly answering these questions. Here we report robust, multi-lineage and serially transplantable dHSC activity from cultured 2-7sp murine embryonic explants (Em-Ex). dHSC are undetectable in 2-7sp YS explants. Additionally, the engraftment from Em-Ex is confined to an emerging CD31+CD45+c-Kit+CD41- population. In sum, our work supports a model in which the embryo, not the YS, is the major source of lifelong definitive hematopoiesis.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saco Vitelino / Células-Tronco Hematopoéticas / Linhagem da Célula / Embrião de Mamíferos / Hematopoese Limite: Animals Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saco Vitelino / Células-Tronco Hematopoéticas / Linhagem da Célula / Embrião de Mamíferos / Hematopoese Limite: Animals Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Estados Unidos