Functional Analysis of Human Hepatocytes Isolated From Chimeric Mouse Liver.
Transplant Proc
; 50(10): 3858-3862, 2018 Dec.
Article
em En
| MEDLINE
| ID: mdl-30577278
ABSTRACT
Chimeric mice with humanized liver were first established by transplanting primary human hepatocytes (PHHs) isolated from a Japanese 27-year-old donor into complementary DNA-urokinase-type plasminogen activator/severe combined immunodeficiency mice. The PHHs from the Japanese donor increased more than 100-fold in the mouse liver, and human hepatocytes purified from the chimeric mouse liver (hcPHs) were successfully transplanted into second-passaged mice. These PHHs and hcPHs can produce human albumin and preserve many liver-specific enzyme genes, which are important for liver function. Interestingly, hepatitis B virus can be infected with these chimeric mice; hepatitis B viral DNA and hepatitis B surface antigen levels were detectable. In conclusion, hcPHs can be an ideal cell source for analysis of human hepatocytes.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Quimeras de Transplante
/
Hepatócitos
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Modelos Animais de Doenças
Limite:
Animals
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Humans
Idioma:
En
Revista:
Transplant Proc
Ano de publicação:
2018
Tipo de documento:
Article