In vitro antiviral activity of Griffithsin against porcine epidemic diarrhea virus.
Virus Genes
; 55(2): 174-181, 2019 Apr.
Article
em En
| MEDLINE
| ID: mdl-30637608
ABSTRACT
Griffithsin is a lectin with potent antiviral activity against enveloped viruses. The objective of this study was to assess Griffithsin's inhibitory effect on porcine epidemic diarrhea virus (PEDV). The results showed that Griffithsin reduced PEDV infection of Vero cells by approximately 82.8%. Moreover, using time-of-addition assays and RT-qPCR, we found that delayed addition of Griffithsin had a weaker inhibitory effect on PEDV than earlier treatment. The mechanism of Griffithsin's action against PEDV involved both preventing viral attachment to host cells and disrupting cell-to-cell transmission; its dual mode of action distinguished Griffithsin from most other antiviral drugs. In conclusion, Griffithsin was identified as a potent PEDV inhibitor and may represent a candidate drug for preventing PEDV infection.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antivirais
/
Infecções por Coronavirus
/
Lectinas de Plantas
/
Vírus da Diarreia Epidêmica Suína
Limite:
Animals
Idioma:
En
Revista:
Virus Genes
Assunto da revista:
BIOLOGIA MOLECULAR
/
VIROLOGIA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China