Asp305Gly mutation improved the activity and stability of the styrene monooxygenase for efficient epoxide production in Pseudomonas putida KT2440.

Tan, Chunlin; Zhang, Xian; Zhu, Zhijing; Xu, Meijuan; Yang, Taowei; Osire, Tolbert; Yang, Shangtian; Rao, Zhiming

Tan, Chunlin; Zhang, Xian; Zhu, Zhijing; Xu, Meijuan; Yang, Taowei; Osire, Tolbert; Yang, Shangtian; Rao, Zhiming.

Afiliação

Tan C; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.
Zhang X; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China. zxshengwu@126.com.
Zhu Z; The School of Digital Media, Jiangnan University, Wuxi, 214122, China.
Xu M; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.
Yang T; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.
Osire T; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.
Yang S; Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, 43210, USA.
Rao Z; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China. raozhm@jiangnan.edu.cn.

Microb Cell Fact ; 18(1): 12, 2019 Jan 24.

Article em En | MEDLINE | ID: mdl-30678678

Assuntos

Compostos de Epóxi/metabolismo; Oxigenases/metabolismo; Pseudomonas putida/enzimologia; Sítios de Ligação; Biocatálise; Escherichia coli/metabolismo; Flavina-Adenina Dinucleotídeo/metabolismo; Meia-Vida; Cinética; Simulação de Acoplamento Molecular; Mutagênese Sítio-Dirigida; NAD/metabolismo; Oxigenases/genética; Estabilidade Proteica; Pseudomonas putida/genética; Proteínas Recombinantes/biossíntese; Proteínas Recombinantes/química; Proteínas Recombinantes/isolamento & purificação; Temperatura

Palavras-chave

Biocatalysis; Epoxide; Site-saturation mutagenesis; Styrene monooxygenase

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oxigenases / Pseudomonas putida / Compostos de Epóxi Idioma: En Revista: Microb Cell Fact Assunto da revista: BIOTECNOLOGIA / MICROBIOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China

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