Adipose-derived stem cells delay muscle atrophy after peripheral nerve injury in the rodent model.
Muscle Nerve
; 59(5): 603-610, 2019 05.
Article
em En
| MEDLINE
| ID: mdl-30681163
ABSTRACT
INTRODUCTION:
Injuries to peripheral nerves cause distal muscle atrophy. The effects of adipose-derived stem cell (ASC) injections into a muscle after injury were examined.METHODS:
A 1.5 cm defect in the rat sciatic nerve was created, resulting in gastrocnemius muscle atrophy. The nerve defect was repaired with autograft; DiR-labeled ASCs were injected into the gastrocnemius immediately postoperatively. Quantitation of gross musculature and muscle fiber area, cell survival, fibrosis, lipid deposition, inflammation, and reconstructive responses were investigated.RESULTS:
ASCs were identified in the muscle at 6 weeks, where injections showed increased muscle mass percentage retained, larger average fiber area, and less overall lipid content accumulated throughout the musculature. Muscles having received ASCs showed increased presence of interlukin-10 and Ki67, and decreased inducible nitric oxide synthase (iNOS).DISCUSSION:
This investigation is suggestive that an ASC injection into denervated muscle post-operatively is able to delay the onset of atrophy. Muscle Nerve 59603-603, 2019.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Nervo Isquiático
/
Células-Tronco
/
Atrofia Muscular
/
Músculo Esquelético
/
Transplante de Células-Tronco
/
Traumatismos dos Nervos Periféricos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Muscle Nerve
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Estados Unidos