Factor VIII/von Willebrand factor protein. Galactose a cryptic determinant of von Willebrand factor activity.
J Clin Invest
; 62(2): 496-9, 1978 Aug.
Article
em En
| MEDLINE
| ID: mdl-307560
ABSTRACT
The normal Factor VIII/von Willebrand factor protein has the ability to agglutinate or aggregate normal platelets in the presence of ristocetin (von Willebrand factor activity). Removal of greater than 95% of the sialic acid from this protein by neuraminidase did not affect the von Willebrand factor or procoagulant activity. However, oxidation of the penultimate galactose of the asialo Factor VIII/von Willebrand factor protein with galactose oxidase resulted in a progressive loss of von Willebrand factor activity with no effect on procoagulant activity. Reduction of the 6-aldehydo intermediate by potassium borohydride caused full regeneration of von Willebrand factor activity. These studies confirm the identification of the intact penultimate galactose moiety as a critical determinant of von Willebrand factor activity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Coagulação Sanguínea
/
Fator VIII
/
Fator de von Willebrand
/
Galactose
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Clin Invest
Ano de publicação:
1978
Tipo de documento:
Article