Radiogenomic Analysis of F-18-Fluorodeoxyglucose Positron Emission Tomography and Gene Expression Data Elucidates the Epidemiological Complexity of Colorectal Cancer Landscape.
Comput Struct Biotechnol J
; 17: 177-185, 2019.
Article
em En
| MEDLINE
| ID: mdl-30809322
ABSTRACT
PURPOSE:
Transcriptomic profiling has enabled the neater genomic characterization of several cancers, among them colorectal cancer (CRC), through the derivation of genes with enhanced causal role and informative gene sets. However, the identification of small-sized gene signatures, which can serve as potential biomarkers in CRC, remains challenging, mainly due to the great genetic heterogeneity of the disease.METHODS:
We developed and exploited an analytical framework for the integrative analysis of CRC datasets, encompassing transcriptomic data and positron emission tomography (PET) measurements. Profiling data comprised two microarray datasets, pertaining biopsy specimen from 30 untreated patients with primary CRC, coupled by their F-18-Fluorodeoxyglucose (FDG) PET values, using tracer kinetic analysis measurements. The computational framework incorporates algorithms for semantic processing, multivariate analysis, data mining and dimensionality reduction.RESULTS:
Transcriptomic and PET data feature sets, were evaluated for their discrimination performance between primary colorectal adenocarcinomas and adjacent normal mucosa. A composite signature was derived, pertaining 12 features 7 genes and 5 PET variables. This compact signature manifests superior performance in classification accuracy, through the integration of gene expression and PET data.CONCLUSIONS:
This work represents an effort for the integrative, multilayered, signature-oriented analysis of CRC, in the context of radio-genomics, inferring a composite signature with promising results for patient stratification.
18F-FDG PET; ACADM, Acyl-Coenzyme A Dehydrogenase; AUC, Area Under the Curve; CCT7, Chaperonin Containing TCP1 Subunit 7; CD44, CD44 Molecule (Indian Blood Group); CRC, Colorectal cancer; Colorectal cancer; DE, Differentially Expressed; FD, Fractal Dimension; FDG, F-18-Fluorodeoxyglucose; GDC, Genomics Data Commons; GEO, Gene Expression Omnibus; GSTP1, Glutathione S-Transferase Pi 1; KIT, Proto-Oncogene Receptor Tyrosine Kinase; Lasso, least absolute shrinkage and selection operator; MFA, Multiple Factor Analysis; Microarray analysis; PCs, Principal Components; PET, Positron Emission Tomography; ROC, Receiver-operator Characteristic curve; Radiogenomics; SUV, Standardized Uptake Value; TCGA; TCGA-COAD, The Cancer Genome Atlas-Colon Adenocarcinoma; Translational bioinformatics
Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Comput Struct Biotechnol J
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Grécia