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Structural basis for transcription initiation by bacterial ECF σ factors.
Li, Lingting; Fang, Chengli; Zhuang, Ningning; Wang, Tiantian; Zhang, Yu.
Afiliação
  • Li L; Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China.
  • Fang C; University of Chinese Academy of Sciences, Beijing, 100049, China.
  • Zhuang N; Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China.
  • Wang T; University of Chinese Academy of Sciences, Beijing, 100049, China.
  • Zhang Y; Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China.
Nat Commun ; 10(1): 1153, 2019 03 11.
Article em En | MEDLINE | ID: mdl-30858373
Bacterial RNA polymerase employs extra-cytoplasmic function (ECF) σ factors to regulate context-specific gene expression programs. Despite being the most abundant and divergent σ factor class, the structural basis of ECF σ factor-mediated transcription initiation remains unknown. Here, we determine a crystal structure of Mycobacterium tuberculosis (Mtb) RNAP holoenzyme comprising an RNAP core enzyme and the ECF σ factor σH (σH-RNAP) at 2.7 Å, and solve another crystal structure of a transcription initiation complex of Mtb σH-RNAP (σH-RPo) comprising promoter DNA and an RNA primer at 2.8 Å. The two structures together reveal the interactions between σH and RNAP that are essential for σH-RNAP holoenzyme assembly as well as the interactions between σH-RNAP and promoter DNA responsible for stringent promoter recognition and for promoter unwinding. Our study establishes that ECF σ factors and primary σ factors employ distinct mechanisms for promoter recognition and for promoter unwinding.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fator sigma / Proteínas de Bactérias / RNA Polimerases Dirigidas por DNA / Regulação Bacteriana da Expressão Gênica / Iniciação da Transcrição Genética / Mycobacterium tuberculosis Tipo de estudo: Prognostic_studies Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fator sigma / Proteínas de Bactérias / RNA Polimerases Dirigidas por DNA / Regulação Bacteriana da Expressão Gênica / Iniciação da Transcrição Genética / Mycobacterium tuberculosis Tipo de estudo: Prognostic_studies Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China