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A New Prevalent Densovirus Discovered in Acari. Insight from Metagenomics in Viral Communities Associated with Two-Spotted Mite (Tetranychus urticae) Populations.
François, Sarah; Mutuel, Doriane; Duncan, Alison B; Rodrigues, Leonor R; Danzelle, Celya; Lefevre, Sophie; Santos, Inês; Frayssinet, Marie; Fernandez, Emmanuel; Filloux, Denis; Roumagnac, Philippe; Froissart, Rémy; Ogliastro, Mylène.
Afiliação
  • François S; DGIMI, Univ Montpellier, INRA, F-34095 Montpellier, France. sarah.francois.dgimi@gmail.com.
  • Mutuel D; DGIMI, Univ Montpellier, INRA, F-34095 Montpellier, France. Doriane.mutuel@inra.fr.
  • Duncan AB; ISEM, Univ Montpellier, CNRS, IRD, EPHE, F-34095 Montpellier, France. alison.duncan@umontpellier.fr.
  • Rodrigues LR; Centre for Ecology, Evolution and Environmental Changes (cE3c), Univ Lisbon, Faculty of Science, P-1749016 Lisbon, Portugal. leonor.rodrigues89@gmail.com.
  • Danzelle C; DGIMI, Univ Montpellier, INRA, F-34095 Montpellier, France. celya.danzelle@icloud.com.
  • Lefevre S; ISEM, Univ Montpellier, CNRS, IRD, EPHE, F-34095 Montpellier, France. celya.danzelle@icloud.com.
  • Santos I; ISEM, Univ Montpellier, CNRS, IRD, EPHE, F-34095 Montpellier, France. sophie.lefevre@umontpellier.fr.
  • Frayssinet M; Centre for Ecology, Evolution and Environmental Changes (cE3c), Univ Lisbon, Faculty of Science, P-1749016 Lisbon, Portugal. inesflsantos@gmail.com.
  • Fernandez E; DGIMI, Univ Montpellier, INRA, F-34095 Montpellier, France. marie.frayssinet@inra.fr.
  • Filloux D; CIRAD, UMR BGPI, F-34398 Montpellier, France. emmanuel.fernandez@cirad.fr.
  • Roumagnac P; BGPI, Univ Montpellier, INRA, CIRAD, Montpellier SupAgro, F-34398 Montpellier, France. emmanuel.fernandez@cirad.fr.
  • Froissart R; CIRAD, UMR BGPI, F-34398 Montpellier, France. denis.filloux@cirad.fr.
  • Ogliastro M; BGPI, Univ Montpellier, INRA, CIRAD, Montpellier SupAgro, F-34398 Montpellier, France. denis.filloux@cirad.fr.
Viruses ; 11(3)2019 03 07.
Article em En | MEDLINE | ID: mdl-30866521
ABSTRACT
Viral metagenomics and high throughput sequence mining have revealed unexpected diversity, and the potential presence, of parvoviruses in animals from all phyla. Among arthropods, this diversity highlights the poor knowledge that we have regarding the evolutionary history of densoviruses. The aim of this study was to explore densovirus diversity in a small arthropod pest belonging to Acari, the two-spotted spider mite Tetranychus urticae, while using viral metagenomics based on virus-enrichment. Here, we present the viromes obtained from T. urticae laboratory populations made of contigs that are attributed to nine new potential viral species, including the complete sequence of a novel densovirus. The genome of this densovirus has an ambisens genomic organization and an unusually compact size with particularly small non-structural proteins and a predicted major capsid protein that lacks the typical PLA2 motif that is common to all ambidensoviruses described so far. In addition, we showed that this new densovirus had a wide prevalence across populations of mite species tested and a genomic diversity that likely correlates with the host phylogeny. In particular, we observed a low densovirus genomic diversity between the laboratory and natural populations, which suggests that virus within-species evolution is probably slower than initially thought. Lastly, we showed that this novel densovirus can be inoculated to the host plant following feeding by infected mites, and circulate through the plant vascular system. These findings offer new insights into densovirus prevalence, evolution, and ecology.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Variação Genética / Densovirus / Tetranychidae / Microbiota Tipo de estudo: Prevalence_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Viruses Ano de publicação: 2019 Tipo de documento: Article País de afiliação: França

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Variação Genética / Densovirus / Tetranychidae / Microbiota Tipo de estudo: Prevalence_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Viruses Ano de publicação: 2019 Tipo de documento: Article País de afiliação: França