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Citrullination Controls Dendritic Cell Transdifferentiation into Osteoclasts.
Krishnamurthy, Akilan; Ytterberg, A Jimmy; Sun, Meng; Sakuraba, Koji; Steen, Johanna; Joshua, Vijay; Tarasova, Nataliya K; Malmström, Vivianne; Wähämaa, Heidi; Réthi, Bence; Catrina, Anca I.
Afiliação
  • Krishnamurthy A; Rheumatology Unit, Karolinska University Hospital, Karolinska Institutet, S-171 76 Stockholm, Sweden.
  • Ytterberg AJ; Rheumatology Unit, Karolinska University Hospital, Karolinska Institutet, S-171 76 Stockholm, Sweden.
  • Sun M; Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77 Stockholm, Sweden; and.
  • Sakuraba K; Rheumatology Unit, Karolinska University Hospital, Karolinska Institutet, S-171 76 Stockholm, Sweden.
  • Steen J; Rheumatology Unit, Karolinska University Hospital, Karolinska Institutet, S-171 76 Stockholm, Sweden.
  • Joshua V; Clinical Research Institute, National Hospital Organisation, Kyushu Medical Center, Fukuoka 810-8563, Japan.
  • Tarasova NK; Rheumatology Unit, Karolinska University Hospital, Karolinska Institutet, S-171 76 Stockholm, Sweden.
  • Malmström V; Rheumatology Unit, Karolinska University Hospital, Karolinska Institutet, S-171 76 Stockholm, Sweden.
  • Wähämaa H; Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77 Stockholm, Sweden; and.
  • Réthi B; Rheumatology Unit, Karolinska University Hospital, Karolinska Institutet, S-171 76 Stockholm, Sweden.
  • Catrina AI; Rheumatology Unit, Karolinska University Hospital, Karolinska Institutet, S-171 76 Stockholm, Sweden.
J Immunol ; 202(11): 3143-3150, 2019 06 01.
Article em En | MEDLINE | ID: mdl-31019059
An increased repertoire of potential osteoclast (OC) precursors could accelerate the development of bone-erosive OCs and the consequent bone damage in rheumatoid arthritis (RA). Immature dendritic cells (DCs) can develop into OCs, however, the mechanisms underlying this differentiation switch are poorly understood. We investigated whether protein citrullination and RA-specific anti-citrullinated protein Abs (ACPAs) could regulate human blood-derived DC-OC transdifferentiation. We show that plasticity toward the OC lineage correlated with peptidyl arginine deiminase (PAD) activity and protein citrullination in DCs. Citrullinated actin and vimentin were present in DCs and DC-derived OCs, and both proteins were deposited on the cell surface, colocalizing with ACPAs binding to the cells. ACPAs enhanced OC differentiation from monocyte-derived or circulating CD1c+ DCs by increasing the release of IL-8. Blocking IL-8 binding or the PAD enzymes completely abolished the stimulatory effect of ACPAs, whereas PAD inhibition reduced steady-state OC development, as well, suggesting an essential role for protein citrullination in DC-OC transdifferentiation. Protein citrullination and ACPA binding to immature DCs might thus promote differentiation plasticity toward the OC lineage, which can facilitate bone erosion in ACPA-positive RA.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoclastos / Artrite Reumatoide / Células Dendríticas Limite: Humans Idioma: En Revista: J Immunol Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Suécia

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoclastos / Artrite Reumatoide / Células Dendríticas Limite: Humans Idioma: En Revista: J Immunol Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Suécia