Plant complex type free N-glycans occur in tomato xylem sap.

ABSTRACT

Free N-glycans (FNGs) are ubiquitous in growing plants. Further, acidic peptideN-glycanase is believed to be involved in the production of plant complex-type FNGs (PCT-FNGs) during the degradation of dysfunctional glycoproteins. However, the distribution of PCT-FNGs in growing plants has not been analyzed. Here, we report the occurrence of PCT-FNGs in the xylem sap of the stem of the tomato plant. Abbreviations RP-HPLC reversed-phase HPLC; SF-HPLC size-fractionation HPLC; PA- pyridylamino; PCT plant complex type; Hex hexose; HexNAc N-acetylhexosamine; Pen pentose; Deoxyhex deoxyhexose; Man D-mannose; GlcNAc N-acetyl-D-glucosamine; Xyl D-xylose; Fuc L-fucose; Lea Lewis a (Galß1-3(Fucα1-4)GlcNAc); PCT plant complex type; M3FX Manα1-6(Manα1-3)(Xylß1-2)Manß1-4GlcNAcß1-4(Fucα1-3)GlcNAc-PA; GN2M3FX GlcNAcß1-2Manα1-6(GlcNAcß1-2Manα1-3)(Xylß1-2)Manß1-4GlcNAcß1-4(Fucα1-3)GlcNAc-PA; (Lea)1GN1M3FX Galß1-3(Fucα1-4)GlcNAc1-2 Manα1-6(GlcNAcß1-2Manα1-3)(Xylß1-2)Manß1-4GlcNAcß1-4(Fucα1-3)GlcNAc-PA or GlcNAc1-2Manα1-6(Galß1-3(Fucα1-4)GlcNAc1-2Manα1-3)(Xylß1-2)Manß1-4GlcNAcß1-4(Fucα1-3)GlcNAc-PA.