Dose-dependent pruritogenic and inflammatory effects of intradermal injections of histamine, compound 48/80 and anti-canine IgE in healthy dogs.
Vet Dermatol
; 30(4): 325-e91, 2019 Aug.
Article
em En
| MEDLINE
| ID: mdl-31038263
ABSTRACT
BACKGROUND:
Scratching behaviours associated with intradermal (i.d.) injection of pruritogens such as histamine and compound 48/80 into the skin of mice and humans is the commonly used model to advance itch research and drug development. The predictive validity of this model is poorly documented in dogs.OBJECTIVES:
To evaluate the dose-dependent effects of pruritogenic substances, each with a different mechanism of action, in healthy dogs. ANIMALS Ten healthy laboratory beagles. METHODS AND MATERIALS All dogs were video-recorded for 30 min post-injection (mpi) of i.d. goat anti-canine IgE (4 and 25 µg/site), histamine and compound 48/80 (50, 100, 200, 400 µg/site); two buffered saline injections served as controls. Two blinded investigators reviewed the pruritic behaviours of all video recordings. Global wheal scores were evaluated at 30 min by a blinded investigator.RESULTS:
All dogs showed wheal and erythema at the pruritogen injection site; global wheal scores at 30 min of each substance significantly increased at all concentrations compared to control (P ≤ 0.05). A blinded evaluation revealed that all pruritogens induced mild acute pruritic behaviours at the site of injection. There was no injection site pain seen in any dog. Compared to controls, injections of pruritogens did not significantly affect the pruritic seconds or occurrence of pruritic episodes for any of the substances. CONCLUSIONS AND CLINICALSIGNIFICANCE:
These preliminary results suggest that i.d. injections of the studied pruritogens can induce cutaneous wheal and flare response in healthy dogs; but inconsistencies occur in the induction of itch, even with the different concentrations of pruritogens.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Prurido
/
Histamina
/
Anticorpos Anti-Idiotípicos
/
P-Metoxi-N-metilfenetilamina
/
Eritema
Tipo de estudo:
Evaluation_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Vet Dermatol
Assunto da revista:
DERMATOLOGIA
/
MEDICINA VETERINARIA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Estados Unidos