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Outbreak of Listeria monocytogenes in South Africa, 2017-2018: Laboratory Activities and Experiences Associated with Whole-Genome Sequencing Analysis of Isolates.
Smith, Anthony M; Tau, Nomsa P; Smouse, Shannon L; Allam, Mushal; Ismail, Arshad; Ramalwa, Ntsieni R; Disenyeng, Bolele; Ngomane, Mimmy; Thomas, Juno.
Afiliação
  • Smith AM; 1 Centre for Enteric Diseases, National Institute for Communicable Diseases, Division of the National Health Laboratory Service, Johannesburg, South Africa.
  • Tau NP; 2 Department of Clinical Microbiology and Infectious Diseases, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
  • Smouse SL; 1 Centre for Enteric Diseases, National Institute for Communicable Diseases, Division of the National Health Laboratory Service, Johannesburg, South Africa.
  • Allam M; 1 Centre for Enteric Diseases, National Institute for Communicable Diseases, Division of the National Health Laboratory Service, Johannesburg, South Africa.
  • Ismail A; 3 Sequencing Core Facility, National Institute for Communicable Diseases, Division of the National Health Laboratory Service, Johannesburg, South Africa.
  • Ramalwa NR; 3 Sequencing Core Facility, National Institute for Communicable Diseases, Division of the National Health Laboratory Service, Johannesburg, South Africa.
  • Disenyeng B; 1 Centre for Enteric Diseases, National Institute for Communicable Diseases, Division of the National Health Laboratory Service, Johannesburg, South Africa.
  • Ngomane M; 1 Centre for Enteric Diseases, National Institute for Communicable Diseases, Division of the National Health Laboratory Service, Johannesburg, South Africa.
  • Thomas J; 1 Centre for Enteric Diseases, National Institute for Communicable Diseases, Division of the National Health Laboratory Service, Johannesburg, South Africa.
Foodborne Pathog Dis ; 16(7): 524-530, 2019 07.
Article em En | MEDLINE | ID: mdl-31062992
ABSTRACT
In South Africa, a progressive increase in listeriosis cases was noted from mid-June 2017, heralding what was to become the world's largest listeriosis outbreak. A total of 1060 cases were reported for the period January 1, 2017 to July 17, 2018. We describe laboratory activities, experiences, and results of whole-genome sequencing (WGS) analysis of Listeria monocytogenes isolates associated with this outbreak. Bacteria were identified using the VITEK-2 COMPACT 15 microbial identification system. WGS was performed using Illumina MiSeq technology. WGS data were analyzed using CLC Genomics Workbench Software and free-to-use on-line analysis tools/pipelines. Multilocus sequence typing (MLST) showed that 91% of clinical isolates were sequence type 6 (ST6), determining that the outbreak was largely associated with L. monocytogenes ST6. Epidemiological and laboratory findings led to investigation of a large ready-to-eat processed meat production facility in South Africa, named Enterprise Foods. L. monocytogenes ST6 was found in environmental sampling swabs of the production facility and in ready-to-eat processed meat products (including polony, a product similar to bologna sausage) manufactured at the facility. ST6 isolates, sourced at the Enterprise Foods production facility and from Enterprise food products, were shown by single nucleotide polymorphism (SNP) analysis to be highly related to clinical isolates; these nonclinical ST6 isolates showed <10 SNP differences when compared to clinical ST6 isolates. Core-genome MLST showed that clinical ST6 isolates and Enterprise-related ST6 isolates had no more than 4 allele differences between each other, suggestive of a high probability of epidemiological relatedness. WGS data interpreted together with epidemiological data concluded that the source of the listeriosis outbreak was ready-to-eat processed meat products manufactured by Enterprise Foods. Listeriosis has now been added to the South African list of mandatory notifiable medical conditions. Surveillance systems have been strengthened to facilitate prevention and early detection of listeriosis outbreaks.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Surtos de Doenças / Doenças Transmitidas por Alimentos / Listeriose / Listeria monocytogenes / Produtos da Carne Tipo de estudo: Prognostic_studies / Risk_factors_studies / Screening_studies Limite: Adolescent / Adult / Aged / Aged80 / Child / Child, preschool / Female / Humans / Infant / Male País/Região como assunto: Africa Idioma: En Revista: Foodborne Pathog Dis Assunto da revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA / PARASITOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: África do Sul

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Surtos de Doenças / Doenças Transmitidas por Alimentos / Listeriose / Listeria monocytogenes / Produtos da Carne Tipo de estudo: Prognostic_studies / Risk_factors_studies / Screening_studies Limite: Adolescent / Adult / Aged / Aged80 / Child / Child, preschool / Female / Humans / Infant / Male País/Região como assunto: Africa Idioma: En Revista: Foodborne Pathog Dis Assunto da revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA / PARASITOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: África do Sul