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Radiosensitivity of colorectal cancer to 90Y and the radiobiological implications for radioembolisation therapy.
Lee, Boon Q; Abbott, Elliot M; Able, Sarah; Thompson, James M; Hill, Mark A; Kartsonaki, Christiana; Vallis, Katherine A; Falzone, Nadia.
Afiliação
  • Lee BQ; CRUK/MRC Oxford Institute for Radiation Oncology, Department of Oncology, University of Oxford, Oxford, United Kingdom. Joint first authors. Author to whom any correspondence should be addressed.
Phys Med Biol ; 64(13): 135018, 2019 07 05.
Article em En | MEDLINE | ID: mdl-31117062
ABSTRACT
Approximately 50% of all colorectal cancer (CRC) patients will develop metastasis to the liver. 90Y selective internal radiation therapy (SIRT) is an established treatment for metastatic CRC. There is still a fundamental lack of understanding regarding the radiobiology underlying the dose response. This study was designed to determine the radiosensitivity of two CRC cell lines (DLD-1 and HT-29) to 90Y ß - radiation exposure, and thus the relative effectiveness of 90Y SIRT in relation to external beam radiotherapy (EBRT). A 90Y-source dish was sandwiched between culture dishes to irradiate DLD-1 or HT-29 cells for a period of 6 d. Cell survival was determined by clonogenic assay. Dose absorbed per 90Y disintegration was calculated using the PENELOPE Monte Carlo code. PENELOPE simulations were benchmarked against relative dose measurements using EBT3 GAFchromic™ film. Statistical regression based on the linear-quadratic model was used to determine the radiosensitivity parameters [Formula see text] and [Formula see text] using R. These results were compared to radiosensitivity parameters determined for 6 MV clinical x-rays and 137Cs γ-ray exposure. Equivalent dose of EBRT in 2 Gy ([Formula see text]) and 10 Gy ([Formula see text]) fractions were derived for 90Y dose. HT-29 cells were more radioresistant than DLD-1 for all treatment modalities. Radiosensitivity parameters determined for 6 MV x-rays and 137Cs γ-ray were equivalent for both cell lines. The [Formula see text] ratio for 90Y ß --particle exposure was over an order of magnitude higher than the other two modalities due to protraction of dose delivery. Consequently, an 90Y SIRT absorbed dose of 60 Gy equates to an [Formula see text] of 28.7 and 54.5 Gy and an [Formula see text] of 17.6 and 19.3 Gy for DLD-1 and HT-29 cell lines, respectively. We derived radiosensitivity parameters for two CRC cell lines exposed to 90Y ß --particles, 6 MV x-rays, and 137Cs γ-ray irradiation. These radiobiological parameters are critical to understanding the dose response of CRC lesions and ultimately informs the efficacy of 90Y SIRT relative to other radiation therapy modalities.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tolerância a Radiação / Radioisótopos de Ítrio / Neoplasias Colorretais / Embolização Terapêutica Tipo de estudo: Health_economic_evaluation Limite: Humans Idioma: En Revista: Phys Med Biol Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tolerância a Radiação / Radioisótopos de Ítrio / Neoplasias Colorretais / Embolização Terapêutica Tipo de estudo: Health_economic_evaluation Limite: Humans Idioma: En Revista: Phys Med Biol Ano de publicação: 2019 Tipo de documento: Article