Protective effects of l-arginine against testosterone synthesis decreased by T-2 toxin in mouse Leydig cells.
Theriogenology
; 134: 98-103, 2019 Aug.
Article
em En
| MEDLINE
| ID: mdl-31158736
ABSTRACT
The testosterone levels decreased by T-2 toxin in mouse Leydig cells were reported previously. It is not known, however, whether l-arginine improves the situation and what's the mechanism. Leydig cells were isolated and cultured with control, 10â¯nM T-2 toxin, 0.25, 0.5 or 1.0â¯mM l-arginine, and 10â¯nM T-2 toxin supplemented with 0.25, 0.5 or 1.0â¯mM l-arginine for 24â¯h. Cells and supernatants were collected to detect the mRNA expression and activities of P450scc (cholesterol side-chain cleavage enzyme), 3ß-HSD-1 (3ß-hydroxysteroid dehydrogenase/isomerase-1) and StAR (steroidogenic acute regulatory protein). Results revealed that l-arginine increased the testosterone levels declined by T-2 toxin and up-regulated the activities and mRNA expression of P450scc, 3ß-HSD-1 and StAR down-regulated by T-2 toxin in Leydig cells. Therefore, we concluded that l-arginine ameliorated the testosterone levels decreased by T-2 Toxin via regulating the mRNA expression and activities of P450scc, 3ß-HSD-1 and StAR in mouse Leydig cells.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arginina
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Toxina T-2
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Testosterona
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Substâncias Protetoras
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Células Intersticiais do Testículo
Limite:
Animals
Idioma:
En
Revista:
Theriogenology
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China