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Development of a reverse genetics system for Japanese encephalitis virus strain SA14-14-2.
Li, Guohua; Jin, Hongli; Nie, Xin; Zhao, Yongkun; Feng, Na; Cao, Zongxi; Tan, Shuyi; Zhang, Bo; Gai, Weiwei; Yan, Feihu; Li, Ling; Zhang, Ying; Cao, Zengguo; Li, Nan; Gao, Yuwei; Yang, Songtao; Xia, Xianzhu; Wang, Hualei.
Afiliação
  • Li G; College of Animal Science and Technology, Shihezi University, Shihezi, 832003, China.
  • Jin H; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, 130122, China.
  • Nie X; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, 130122, China.
  • Zhao Y; Key laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, 130062, China.
  • Feng N; 65316 Troops, Peoples' Liberation Army, Wafangdian, 116300, China.
  • Cao Z; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, 130122, China.
  • Tan S; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University, Yangzhou, 225009, China.
  • Zhang B; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, 130122, China.
  • Gai W; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University, Yangzhou, 225009, China.
  • Yan F; Hainan Academician Workstation, Institute of Animal Husbandry and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou, 571100, China.
  • Li L; Hainan Academician Workstation, Institute of Animal Husbandry and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou, 571100, China.
  • Zhang Y; Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan, 430071, China.
  • Cao Z; Key laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, 130062, China.
  • Li N; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, 130122, China.
  • Gao Y; Key laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, 130062, China.
  • Yang S; National Research Center for Exotic Animal Disease, Animal Health and Epidemiology Center, Qingdao, 266032, China.
  • Xia X; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, 130122, China.
  • Wang H; College of Animal Science and Technology, Jilin Agricutural University, Changchun, 130118, China.
Virus Genes ; 55(4): 550-556, 2019 Aug.
Article em En | MEDLINE | ID: mdl-31161411
ABSTRACT
Japanese encephalitis virus SA14-14-2 (JEV SA14-14-2) is a widely used vaccine in China and other southeastern countries to prevent Japanese encephalitis in children. In this study, a stable infectious cDNA clone of JEV SA14-14-2 with a low copy number pACYC177 vector dependent on the T7 promoter and T7 terminator was developed. Two introns were inserted into the capsid gene and envelope gene of JEV cDNA for gene stability. Hepatitis delta virus ribozyme (HDVr) was engineered into the 3' UTR cDNA of JEV for authentic 3' UTR transcription. The rescued virus showed biological properties indistinguishable from those of the parent strain (JEV SA14-14-2). The establishment of a JEV SA14-14-2 reverse genetics system lays the foundation for the further development of other flavivirus vaccines and viral pathogenesis studies.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírus da Encefalite Japonesa (Espécie) / Genética Reversa Idioma: En Revista: Virus Genes Assunto da revista: BIOLOGIA MOLECULAR / VIROLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírus da Encefalite Japonesa (Espécie) / Genética Reversa Idioma: En Revista: Virus Genes Assunto da revista: BIOLOGIA MOLECULAR / VIROLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China