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Molecular Epidemiology of Ralstonia solanacearum Species Complex Strains Causing Bacterial Wilt of Potato in Uganda.
Abdurahman, Abdulwahab; Parker, Monica L; Kreuze, Jan; Elphinstone, John G; Struik, Paul C; Kigundu, Andrew; Arengo, Esther; Sharma, Kalpana.
Afiliação
  • Abdurahman A; Consultative Group for International Agricultural Research, Research Program on Roots, Tubers and Bananas, Lima, Peru.
  • Parker ML; Sub-Saharan Africa Regional Office, International Potato Center, Nairobi, Kenya.
  • Kreuze J; Centre for Crop Systems Analysis, Plant Sciences, Wageningen University and Research, Wageningen, The Netherlands.
  • Elphinstone JG; Consultative Group for International Agricultural Research, Research Program on Roots, Tubers and Bananas, Lima, Peru.
  • Struik PC; Sub-Saharan Africa Regional Office, International Potato Center, Nairobi, Kenya.
  • Kigundu A; Consultative Group for International Agricultural Research, Research Program on Roots, Tubers and Bananas, Lima, Peru.
  • Arengo E; Crop and Systems Sciences Division, International Potato Center, Lima, Peru.
  • Sharma K; National Agri-Food Innovation Campus, Fera Science Ltd, Sand Hutton, York, United Kingdom.
Phytopathology ; 109(11): 1922-1931, 2019 Nov.
Article em En | MEDLINE | ID: mdl-31272278
ABSTRACT
Bacterial wilt (BW) caused by the Ralstonia solanacearum species complex (RSSC) is a serious threat to potato production in Uganda. However, little is known about the extent of the disease and the type of the pathogen strains involved. A nationwide survey was conducted to study BW prevalence and incidence in potato, and potato tuber and stem samples of potential alternative hosts were collected for pathogen isolation. DNA was extracted from pure cultures for genetic diversity studies. The pathogen was phylotyped by multiplex PCR; then, a subset of isolates was typed at sequevar level. Isolates of the same sequevar were then haplotyped using multilocus tandem repeat sequence typing (TRST) schemes. BW prevalence and incidence in potato farms were 81.4 and 1.7%, respectively. Three RSSC phylotypes were identified, with the majority of the strains belonging to Phylotype II (80%) followed by Phylotype I (18.5%) and III (1.5%). Phylotype I strains belonged to Sequevar 31, and Phylotype II strains belonged to Sequevar 1. Potato-associated Phylotype II Sequevar 1 strains were more diverse (27 TRST haplotypes) than nonpotato Phylotype I (5 TRST haplotypes). Mapping of TRST haplotypes revealed that three TRST haplotypes of Phylotype II Sequevar 1 strains play an important epidemiological role in BW of potato in Uganda being disseminated via latently infected seed.[Formula see text]Copyright © 2019 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Solanum tuberosum / Epidemiologia Molecular / Ralstonia solanacearum Tipo de estudo: Risk_factors_studies / Screening_studies País/Região como assunto: Africa Idioma: En Revista: Phytopathology Assunto da revista: BOTANICA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Peru

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Solanum tuberosum / Epidemiologia Molecular / Ralstonia solanacearum Tipo de estudo: Risk_factors_studies / Screening_studies País/Região como assunto: Africa Idioma: En Revista: Phytopathology Assunto da revista: BOTANICA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Peru