Complementary techniques (spICP-MS, TEM, and HPLC-ICP-MS) reveal the degradation of 40 nm citrate-stabilized Au nanoparticles in rat liver after intraperitoneal injection.
J Trace Elem Med Biol
; 55: 1-5, 2019 Sep.
Article
em En
| MEDLINE
| ID: mdl-31345346
ABSTRACT
BACKGROUND:
Due to the increased use of engineered nanoparticles (NPs), their tracing in environmental and biological systems is of utmost importance. Besides their accumulation within a biological specimen, little is known about their degradation and transformation into corresponding low-molecular species that might influence any toxicological impact. ANALYTICALMETHODS:
Wistar rats underwent intraperitoneal injections of 40 nm citrate-stabilized gold nanoparticles. Different liver samples were analysed for the occurrence of nanoparticles and potential degradation products by means of spICP-MS, TEM and HPLC-ICP-MS. MAINFINDINGS:
Studies using spICP-MS revealed the presence of the originally administrated Au NPs (40 nm diameter) and some evidences of other Au-containing species due to the increased background signal. Images obtained by transmission electron microscopy (TEM) showed the predominant presence of particles of significantly smaller diameter (6 ± 2 nm). As complementary method, HPLC-ICP-MS confirmed the presence of both particle types indicating a degradation of the Au NPs accompanied by detection of low-molecular Au species.CONCLUSIONS:
This study underlines that degradation of gold nanoparticles to low-molecular gold species might have to be taken into account in future for studies on their toxicological behaviour and their potential use in clinical applications.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácido Cítrico
/
Nanopartículas Metálicas
/
Ouro
/
Fígado
Limite:
Animals
Idioma:
En
Revista:
J Trace Elem Med Biol
Assunto da revista:
METABOLISMO
/
SAUDE AMBIENTAL
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Espanha